Biotin Anti-TPO 抗体 (ab271257)
Key features and details
- Biotin Rabbit polyclonal to TPO
- Suitable for: WB, ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-TPO antibody -
製品の詳細
Biotin Rabbit polyclonal to TPO -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human TPO aa 1-200.
Database link: P40225 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab271257の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 38 kDa.
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ELISA |
Use a concentration of 0.25 - 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 38 kDa. |
ELISA
Use a concentration of 0.25 - 1 µg/ml. |
ターゲット情報
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関連性
TPO acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Additional splice variants have been described but their biological natures have not been determined. -
細胞内局在
Secreted -
別名
- c-mpl ligand antibody
- megakaryocyte colony-stimulating factor antibody
- megakaryocyte growth and development factor antibody
see all
画像
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Western blot analysis of varying levels of Recombinant human TPO protein using ab271257 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant TPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24 -
Human TPO was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271257. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant TPO protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab271257 は論文での使用が確認できていません。