Biotin Anti-TPO 抗体 (ab271257)
Key features and details
- Biotin Rabbit polyclonal to TPO
- Suitable for: WB, ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-TPO antibody -
製品の詳細
Biotin Rabbit polyclonal to TPO -
由来種
Rabbit -
標識
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentWB Recombinant fragment
-
免疫原
Recombinant fragment corresponding to Human TPO aa 1-200.
Database link: P40225
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ELISA |
Recombinant fragment
|
| WB |
Recombinant fragment
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 38 kDa.
|
|
| ELISA |
Use a concentration of 0.25 - 1 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 38 kDa. |
|
ELISA
Use a concentration of 0.25 - 1 µg/ml. |
ターゲット情報
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関連性
TPO acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Additional splice variants have been described but their biological natures have not been determined. -
細胞内局在
Secreted -
別名
- c-mpl ligand antibody
- megakaryocyte colony-stimulating factor antibody
- megakaryocyte growth and development factor antibody
see all
画像
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Western blot - Biotin Anti-TPO antibody (ab271257)
Western blot analysis of varying levels of Recombinant human TPO protein using ab271257 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant TPO is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24 -
ELISA - Biotin Anti-TPO antibody (ab271257)Human TPO was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271257. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant TPO protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271257 は論文での使用が確認できていません。
