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Biotin Anti-TLR4 抗体 [MTS510] (ab9105)

Submit a review Q&A (2)References (2)

Key features and details

  • Biotin Rat monoclonal [MTS510] to TLR4
  • Suitable for: IHC-Fr, IP, Flow Cyt, Functional Studies
  • Reacts with: Mouse
  • Conjugation: Biotin
  • Isotype: IgG2a

製品の概要

  • 製品名

    Biotin Anti-TLR4 antibody [MTS510]
    TLR4 一次抗体 製品一覧

  • 製品の詳細

    Biotin Rat monoclonal [MTS510] to TLR4

  • 由来種

    Rat

  • 標識

    Biotin

  • 特異性

    Only detects murine TLR-4 when complexed to MD-2. This antibody has been found to coprecipitate MD-2 (30kDa) with TLR4 (100kDa).

  • アプリケーション

    適用あり: IHC-Fr, IP, Flow Cyt, Functional Studiesmore details

  • 種交差性

    交差種: Mouse

  • 免疫原

    Tissue, cells or virus corresponding to TLR4. Mouse Ba/F3 cells expressing mouse TLR4

  • 特記事項


    Ab9105 is a TLR4 function blocking antibody that is useful for studies on the role of TLR4 as a receptor fpr LPS induced cytokine production by TLR4 bearing cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9105の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Functional Studies
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Functional Studies
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.

  • 組織特異性

    Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

  • 関連疾患

    Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.

  • 配列類似性

    Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.

  • ドメイン

    The TIR domain mediates interaction with NOX4.

  • 翻訳後修飾

    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ARMD10 antibody
      • CD284 antibody
      • CD284 antigen antibody
      • Homolog of Drosophila toll antibody
      • hToll antibody
      • TLR 4 antibody
      • TLR4 antibody
      • TLR4_HUMAN antibody
      • TOLL antibody
      • Toll like receptor 4 antibody
      • Toll-like receptor 4 antibody
      see all

    参考文献 (2)

    ab9105 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9105 は 2 報の論文で使用されています。

    • Chakraborty D  et al. Alarmin S100A8 Activates Alveolar Epithelial Cells in the Context of Acute Lung Injury in a TLR4-Dependent Manner. Front Immunol 8:1493 (2017). PubMed: 29180999
    • Daubeuf B  et al. TLR4/MD-2 monoclonal antibody therapy affords protection in experimental models of septic shock. J Immunol 179:6107-14 (2007). Flow Cyt ; Mouse . PubMed: 17947685

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    Has this antibody been tested to determine whether it can block LPS induced upregulation of co-stimulatory molecules (e.g cd86)

    Read More
    Answer

    Thank you for your enquiry. ab9105 is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to co precipitate MD-2 (30kDa) with TLR4 (100 kDa). The blocking activity of this antibody has been described in the following article: Akashi, S et al; Cutting edge: Cell surface expression and lipopolysaccharide signaling via the toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages. J Immunol 2000, 164: 3471 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    Could you tell me if your antibody against TRL4/MD2 complex, TLR4 antibody [MTS510], could bind to MD-2 alone?

    Read More
    Answer

    Thank you for your enquiry. The antibody recognizes the mouse TLR4/MD2 complex and is not useful for detection of TLR4 or MD2 alone.

    Read More

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