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Biotin Anti-SLPI 抗体 [31] (ab17158)

Submit a review Q&A (1)References (1)

Key features and details

  • Biotin Mouse monoclonal [31] to SLPI
  • Suitable for: IHC-Fr, IHC-P, WB
  • Reacts with: Human
  • Conjugation: Biotin
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Biotin Anti-SLPI antibody [31]
    SLPI 一次抗体 製品一覧

  • 製品の詳細

    Biotin Mouse monoclonal [31] to SLPI

  • 由来種

    Mouse

  • 標識

    Biotin

  • 特異性

    The reactivity of the antibody with the isolated domains was evaluated using isolated domains of SLPI obtained by cleavage using partial acidic hydrolysis. Therefore, it is possible that the antibody will recognize other SLPI cleavage products generated by e.g. (microbial) proteases.

  • アプリケーション

    適用あり: IHC-Fr, IHC-P, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Full length native protein (purified) corresponding to SLPI. Purified human antileukoprotease (ALP)

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab17158の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
特記事項
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G. May prevent elastase-mediated damage to oral and possibly other mucosal tissues.

  • 組織特異性

    Mucous fluids.

  • 配列類似性

    Contains 2 WAP domains.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ALK 1 antibody
      • ALK1 antibody
      • ALP antibody
      • Antileukoprotease antibody
      • Antileukoproteinase 1 antibody
      • Antileukoproteinase antibody
      • Antileukoproteinase1 antibody
      • BLPI antibody
      • Human seminal protease inhibitor antibody
      • HUSI 1 antibody
      • HUSI antibody
      • HUSI I antibody
      • HUSI-1 antibody
      • MPI antibody
      • Mucus proteinase inhibitor antibody
      • Protease inhibitor WAP4 antibody
      • Secretory leukocyte peptidase inhibitor antibody
      • Secretory leukocyte protease inhibitor antibody
      • Seminal proteinase inhibitor antibody
      • SLPI antibody
      • SLPI_HUMAN antibody
      • WAP four disulfide core domain protein 4 antibody
      • WAP four-disulfide core domain protein 4 antibody
      • WAP4 antibody
      • WFDC4 antibody
      see all

    参考文献 (1)

    ab17158 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab17158 は 1 報の論文で使用されています。

    • Skrzeczynska-Moncznik J  et al. Secretory leukocyte proteinase inhibitor-competent DNA deposits are potent stimulators of plasmacytoid dendritic cells: implication for psoriasis. J Immunol 189:1611-7 (2012). IHC ; Human . PubMed: 22786767

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 121940 DESCRIPTION OF THE PROBLEM In the description of the biotinylated antibody against SLPI it states that the antibody does not recognize cleaved portions or domains of the protein, only the intact protein. I have found that this particular antibody recognizes both intact and cleaved protein under reducing and non-reducing conditions the same as the non-biotinylated antibody. I would love to find out how you were able to get the antibody to react differently to the two different species of protein as I would like to design an assay around that functionality. SAMPLE Purified SLPI protein (recombinant) from R&D Systems. PRIMARY ANTIBODY anti-SLPI biotin or anti-SLPI purified (Abcam [31]) DETECTION METHOD ECL (Pierce) POSITIVE AND NEGATIVE CONTROLS USED Undigested protein, no protein, markers, ANTIBODY STORAGE CONDITIONS 4oC refrigerator in the dark SAMPLE PREPARATION 1mg/ml in 1X PBS AMOUNT OF PROTEIN LOADED 20ug ELECTROPHORESIS/GEL CONDITIONS Many conditions, all show same result. TRANSFER AND BLOCKING CONDITIONS Invitrogen NuPAGE system SECONDARY ANTIBODY anti-mouse=HRP, or Streptavidin=HRP HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Reducing vs. Non-reducing BME vs. DTT Tris-glycine/MES vs. TRICINE ADDITIONAL NOTES Good luck, we're all counting on you...

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    Answer

    We forwarded your question to an expert in this field and this is the answer I received: The reactivity of the antibody with the isolated domains was evaluated using isolated domains of SLPI obtained by cleavage using partial acidic hydrolysis. Therefore, it is entirely possible that the antibody does recognize other SLPI cleavage products generated by e.g. (microbial) proteases. I have removed the sentence on the datasheet which originated from a customer feedback and hope this information helps you understand your results. I'm very sorry this sentence was not correct.

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