Biotin Anti-Resistin 抗体 (ab271218)
Key features and details
- Biotin Rabbit polyclonal to Resistin
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-Resistin antibody
Resistin 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to Resistin -
由来種
Rabbit -
標識
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA Recombinant fragmentWB Recombinant fragment
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免疫原
Recombinant fragment corresponding to Human Resistin aa 1-150.
Database link: Q9HD89 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| sELISA |
Recombinant fragment
|
| WB |
Recombinant fragment
|
| All applications |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa.
|
|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa. |
|
Sandwich ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. -
組織特異性
Expressed only in fatty tissues. -
配列類似性
Belongs to the resistin/FIZZ family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 56729 Human
- Omim: 605565 Human
- SwissProt: Q9HD89 Human
- Unigene: 283091 Human
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別名
- Adipose tissue specific secretory factor antibody
- Adipose tissue-specific secretory factor antibody
- ADSF antibody
see all
画像
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Western blot - Biotin Anti-Resistin antibody (ab271218)
To detect Human Resistin Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Resistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human Resistin, respectively.
Non-reducing conditions.
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Sandwich ELISA - Biotin Anti-Resistin antibody (ab271218)Human resistin was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271218.
This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant human resistin protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271218 は論文での使用が確認できていません。