Biotin Anti-RELM beta 抗体 (ab271225)
Key features and details
- Biotin Rabbit polyclonal to RELM beta
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-RELM beta antibody
RELM beta 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to RELM beta -
由来種
Rabbit -
標識
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA Recombinant fragmentWB Recombinant fragment
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免疫原
Recombinant fragment corresponding to Mouse RELM beta aa 1 to the C-terminus.
Database link: Q99P86
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| sELISA |
Recombinant fragment
|
| WB |
Recombinant fragment
|
| All applications |
Mouse
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa.
|
|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa. |
|
Sandwich ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Probable hormone. -
組織特異性
Expressed only in the gastrointestinal tract, particularly the colon. -
配列類似性
Belongs to the resistin/FIZZ family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 57263 Mouse
- SwissProt: Q99P86 Mouse
- Unigene: 21123 Mouse
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別名
- C/EBP epsilon regulated myeloid specific secreted cysteine rich protein precursor 2 antibody
- CCGR antibody
- Colon and small intestine specific cysteine rich protein antibody
see all
画像
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Western blot - Biotin Anti-RELM beta antibody (ab271225)
To detect mouse RELM beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant mouse RELM beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse RELM beta, respectively.
Reducing conditions.
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Sandwich ELISA - Biotin Anti-RELM beta antibody (ab271225)Mouse RELMβ was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271225.
This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mouse RELMβ protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271225 は論文での使用が確認できていません。
