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Biotin Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18445)

Q&A (1)References (3)

Conjugates logo Related conjugates and formulations

FITC Unconjugated

製品の概要

  • 製品名

    Biotin Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control
    Rat アイソタイプ・コントロール 製品一覧

  • 標識

    Biotin

  • 特記事項（標識）

    ab18445 is conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

  • アプリケーション

    適用あり: Flow Cytmore details

  • 免疫原

    Trinitrophenol + KLH

  • 特記事項


    ab18445 RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    ab18445 was purified by affinity chromatography,

  • アイソタイプ・コントロール 備考

    ab18445 RTK2758 immunoglobulin is useful as an isotype-matched control in Western blotting, immunoprecipitation, immunohistochemical staining, bioassay, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

  • ポリ/モノ

    モノクローナル

  • クローン名

    RTK2758

  • アイソタイプ

    IgG2a

  • 軽鎖の種類

    kappa

  • 研究分野

  • 別名

    • Rat Isotype Control

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab18445の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent dilution. Surface or intracellular staining.
特記事項
Flow Cyt
Use at an assay dependent dilution. Surface or intracellular staining.

参考文献 (3)

ab18445 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab18445 は 3 報の論文で使用されています。

  • Akiyama S  et al. Th17 cells increase during maturation in peripheral blood of healthy dogs. Vet Immunol Immunopathol 209:17-21 (2019). PubMed: 30885301
  • Zeng SL  et al. Mesenchymal stem cells abrogate experimental asthma by altering dendritic cell function. Mol Med Rep 12:2511-20 (2015). Mouse . PubMed: 25936350
  • Yan F  et al. Down-regulation of extracellular signal-regulated kinase 1/2 activity in P-glycoprotein-mediated multidrug resistant hepatocellular carcinoma cells. World J Gastroenterol 15:1443-51 (2009). Flow Cyt . PubMed: 19322916

レビューと Q&A

Q&A
質問を送る

Question

We are customers of abcam antibody products. We recently purchased ABCAM CN:ab18445 200ug (0.5mg/mL) Rat IgG2a-Biotin LN: GR76161-2

And ABCAM CN:ab37394 200ug (0.5mg/mL) Goat F(ab')2 IgG-Fitc LN: GR41305-1.

On 28Nov12 we had a temperature excursion on our 2-8c Refrigerator which recorded -20c for 4 hrs. We use this product to test all our final products. So we have a Deviation to record and to justify the antibody performance.

Could you please provide any information on the stability of the product after freeze and thaw. I would greatly appreciate of any information.

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Answer

Thank you for contacting us.

We do not have any stability testing data for either of these antibodies. In general, it is recommended to avoid freezing either of these antibodies but it is expected that a single freeze-thaw cycle will not affect the performance of either antibody. Please note that the lot of ab37394 that you have expired in August. (Our records show it was purchased in June 2011). It is possible it may still perform adequately, however. The lot of ab18445 that you have, GR76161-2, expires in May 2013.

Please contact us if you have any more questions.

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