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Biotin Mouse IgG2a, kappa [MOPC-173] - Isotype Control (ab18454)

Q&A (1)

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 APC FITC PerCP Unconjugated

製品の概要

  • 製品名

    Biotin Mouse IgG2a, kappa [MOPC-173] - Isotype Control
    Mouse アイソタイプ・コントロール 製品一覧

  • 標識

    Biotin

  • 特異性

    ab18454 MOPC-173 immunoglobulin has unknown specificity. ab18454 MOPC-173 immunoglobulin was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues.

  • アプリケーション

    適用あり: Flow Cytmore details

  • 特記事項

    ab18454 MOPC-173 immunoglobulin is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • 特記事項（精製）

    ab18454 antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.

  • アイソタイプ・コントロール 備考

    ab18454 MOPC-173 immunoglobulin is useful as an isotype-matched control for Western blotting, immunoprecipitation, immunohistochemical staining, and immunofluorescent staining (surface or intracellular) for flow cytometric analysis.

  • ポリ/モノ

    モノクローナル

  • クローン名

    MOPC-173

  • アイソタイプ

    IgG2a

  • 軽鎖の種類

    kappa

  • 研究分野

  • 別名

    • Mouse Isotype Control

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab18454の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.
特記事項
Flow Cyt
Use at an assay dependent concentration.

Suitable for surface or intracellular staining.

参考文献 (0)

ab18454 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab18454 は論文での使用が確認できていません。

レビューと Q&A

Q&A
質問を送る

Question

Dear Abcam,
I would like to ask a question about which control IgG I need to use for
28-14-8 (biotin) (#ab25237)
I want to use same isotype with biotin conjugated antibody and I found that ab 128976, which is biotinylated
Goat anti-Mouse IgG, but I am not sure if this is the right one to use.
Your kind help would be much appreciated.
Thanks a lot.

Read More
Answer

Thank you for contacting us.

I wouldn't recommend using ab128976 as an isotype control with ab25237 since ab128976 is raised in a goat and it needs to match the primary antibody you're using.

I would recommend ab18454 as an isotype control since this matches the species, isotype, clonality and conjugation of the primary antibody you're using.

https://www.abcam.com/Mouse-IgG2a-kappa-monoclonal-MOPC-173-biotin-Isotype-control-ab18454.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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