Biotin Anti-MIP-3 beta/CCL19 抗体 (ab271285)
Key features and details
- Biotin Rabbit polyclonal to MIP-3 beta/CCL19
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-MIP-3 beta/CCL19 antibody
MIP-3 beta/CCL19 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to MIP-3 beta/CCL19 -
由来種
Rabbit -
標識
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA Recombinant fragmentWB Recombinant fragment
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免疫原
Recombinant full length protein corresponding to Human MIP-3 beta/CCL19 aa 1 to the C-terminus (biotinylated ). Mature chain. E. coli derived.
Database link: Q99731 -
ポジティブ･コントロール
- WB: Recombinant human MIP-3 beta/CCL19.
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| sELISA |
Recombinant fragment
|
| WB |
Recombinant fragment
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3 beta/CCL19 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human MIP-3 beta/CCL19 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
Sandwich ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. Specifically binds to chemokine receptor CCR7. Recombinant CCL19 shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes. -
組織特異性
Expressed at high levels in the lymph nodes, thymus and appendix. Intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Beta chemokine exodus 3 antibody
- Beta-chemokine exodus-3 antibody
- C C chemokine ligand 19 antibody
see all
画像
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Western blot - Biotin Anti-MIP-3 beta/CCL19 antibody (ab271285)
Lane 1: Marker.
Lane 2: Recombinant hMIP-3 beta/CCL19 at 250ng/lane.
Lane 3: Recombinant hMIP-3 beta/CCL19 at 125ng/lane.
Lane 4: Recombinant hMIP-3 beta/CCL19 at 62.5ng/lane.
Lane 5: Recombinant hMIP-3 beta/CCL19 at 31.25ng/lane.
Lane 6: Recombinant hMIP-3 beta/CCL19 at 15.625ng/lane.
Lane 7: Recombinant hMIP-3 beta/CCL19 at 7.8ng/lane.
Lane 8: Recombinant hMIP-3 beta/CCL19 at 3.9ng/lane.
Lane 9: Recombinant hMIP-3 beta/CCL19 at 1.95ng/lane.
Lane 10: Recombinant hMIP-3 beta/CCL19 at 0.975ng/lane.
Lane 11: Recombinant hMIP-3 beta/CCL19 at 0.4875ng/lane.
Lane 12: Recombinant hMIP-3 beta/CCL19 at 0.24ng/lane. -
Sandwich ELISA - Biotin Anti-MIP-3 beta/CCL19 antibody (ab271285)Human MIP-3 beta/CCL19 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271285. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant MIP-3 beta/CCL19 protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271285 は論文での使用が確認できていません。