Biotin Anti-MCP4 抗体 (ab271197)
Key features and details
- Biotin Rabbit polyclonal to MCP4
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-MCP4 antibody
MCP4 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to MCP4 -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: WB, Sandwich ELISAmore details -
種交差性
交差種: Recombinant fragment
交差が予測される動物種: Human -
免疫原
Recombinant fragment corresponding to Human MCP4 aa 1-100.
Database link: Q99616 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab271197の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa.
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Sandwich ELISA |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa. |
Sandwich ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. -
組織特異性
Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
翻訳後修飾
One major form (form long), and two minor forms (short chain and medium chain) are produced by differential signal peptide cleavage. The medium chain is about 30-fold less active than the long chain. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 6357 Human
- Omim: 601391 Human
- SwissProt: Q99616 Human
- Unigene: 414629 Human
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別名
- C-C motif chemokine 13 antibody
- CCL13 antibody
- CCL13_HUMAN antibody
see all
画像
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To detect Human MCP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml.
When used in conjunction with compatible development reagents the detection limit for Recombinant Human MCP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human MCP4, respectively.
Reducing conditions.
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Human MCP-4 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271197.
This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 2000-4000 pg/ml of recombinant human MCP-4 protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab271197 は論文での使用が確認できていません。