Biotin Anti-Macrophage Inflammatory Protein 3 alpha 抗体 (ab271284)
Key features and details
- Biotin Rabbit polyclonal to Macrophage Inflammatory Protein 3 alpha
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-Macrophage Inflammatory Protein 3 alpha antibody
Macrophage Inflammatory Protein 3 alpha 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to Macrophage Inflammatory Protein 3 alpha -
由来種
Rabbit -
標識
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA Recombinant fragmentWB Recombinant fragment
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免疫原
Recombinant full length protein corresponding to Human Macrophage Inflammatory Protein 3 alpha aa 1 to the C-terminus. Mature chain. E. coli derived.
Database link: P78556 -
ポジティブ･コントロール
- WB: Recombinant human Macrophage Inflammatory Protein 3 alpha.
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| sELISA |
Recombinant fragment
|
| WB |
Recombinant fragment
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 10 kDa.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant human Macrophage Inflammatory Protein 3 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 10 kDa. When used in conjunction with compatible secondary reagents, the detection limit for recombinant human Macrophage Inflammatory Protein 3 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
Sandwich ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. -
組織特異性
Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
翻訳後修飾
C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Beta chemokine exodus 1 antibody
- Beta-chemokine exodus-1 antibody
- C C motif chemokine ligand 20 antibody
see all
画像
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Western blot - Biotin Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab271284)
Lane 1: Marker.
Lane 2: Recombinant hMIP3 alpha at 250ng/lane.
Lane 3: Recombinant hMIP3 alpha at 125ng/lane.
Lane 4: Recombinant hMIP3 alpha at 62.5ng/lane.
Lane 5: Recombinant hMIP3 alpha at 31.25ng/lane.
Lane 6: Recombinant hMIP3 alpha at 15.625ng/lane.
Lane 7: Recombinant hMIP3 alpha at 7.8ng/lane.
Lane 8: Recombinant hMIP3 alpha at 3.9ng/lane.
Lane 9: Recombinant hMIP3 alpha at 1.95ng/lane.
Lane 10: Recombinant hMIP3 alpha at 0.975ng/lane.
Lane 11: Recombinant hMIP3 alpha at 0.4875ng/lane.
Lane 12: Recombinant hMIP3 alpha at 0.24ng/lane. -
Sandwich ELISA - Biotin Anti-Macrophage Inflammatory Protein 3 alpha antibody (ab271284)Human Macrophage Inflammatory Protein 3 alpha was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271284. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Macrophage Inflammatory Protein 3 alpha protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271284 は論文での使用が確認できていません。
