Biotin Anti-M-CSF/CSF1 抗体 (ab271262)
Key features and details
- Biotin Goat polyclonal to M-CSF/CSF1
- Suitable for: WB, Sandwich ELISA
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-M-CSF/CSF1 antibody -
製品の詳細
Biotin Goat polyclonal to M-CSF/CSF1 -
由来種
Goat -
標識
Biotin -
アプリケーション
適用あり: WB, Sandwich ELISAmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment corresponding to Mouse M-CSF/CSF1 aa 1-200.
Database link: P07141
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 60 kDa.
|
|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 60 kDa. |
|
Sandwich ELISA
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance. -
細胞内局在
Cell membrane; Single-pass membrane protein -
参照データベース
- Entrez Gene: 12977 Mouse
- SwissProt: P07141 Mouse
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別名
- Colony stimulating factor 1 (macrophage) antibody
- CSF-1 antibody
- CSF1 antibody
see all
画像
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Western blot - Biotin Anti-M-CSF/CSF1 antibody (ab271262)
Western blot analysis of varying levels of Recombinant murine M-CSF protein using ab271262 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant M-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24 -
Sandwich ELISA - Biotin Anti-M-CSF/CSF1 antibody (ab271262)Mouse M-CSF was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271262. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mM-CSF protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271262 は論文での使用が確認できていません。
