Biotin Anti-Lymphotactin/ATAC 抗体 (ab271254)
Key features and details
- Biotin Rabbit polyclonal to Lymphotactin/ATAC
- Suitable for: WB, ELISA
- Reacts with: Human, Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-Lymphotactin/ATAC antibody
Lymphotactin/ATAC 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to Lymphotactin/ATAC -
由来種
Rabbit -
標識
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species ELISA HumanWB Recombinant fragment
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免疫原
Recombinant fragment corresponding to Human Lymphotactin/ATAC aa 1 to the C-terminus.
Database link: P47992 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ELISA |
Human
|
| WB |
Recombinant fragment
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 13 kDa.
|
|
| ELISA |
Use a concentration of 0.25 - 1 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 13 kDa. |
|
ELISA
Use a concentration of 0.25 - 1 µg/ml. |
ターゲット情報
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機能
Chemotactic activity for lymphocytes but not for monocytes or neutrophils. -
組織特異性
Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine. -
配列類似性
Belongs to the intercrine gamma family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 6375 Human
- Omim: 600250 Human
- SwissProt: P47992 Human
- Unigene: 546295 Human
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別名
- ATAC antibody
- C motif chemokine 1 antibody
- Chemokine (C motif) ligand 1 antibody
see all
画像
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Western blot - Biotin Anti-Lymphotactin/ATAC antibody (ab271254)
Western blot analysis of varying levels of Recombinant human Lymphotactin protein using ab271254 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant Lymphotactin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24 -
ELISA - Biotin Anti-Lymphotactin/ATAC antibody (ab271254)Human Lymphotactin/ATAC was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271254. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Lymphotactin/ATAC protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271254 は論文での使用が確認できていません。