Biotin Anti-Leptin 抗体 (ab271278)
Key features and details
- Biotin Rabbit polyclonal to Leptin
- Suitable for: WB, ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
-
製品名
Biotin Anti-Leptin antibody
Leptin 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to Leptin -
由来種
Rabbit -
標識
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentWB Recombinant fragment
-
免疫原
Recombinant full length protein corresponding to Human Leptin aa 1 to the C-terminus. Mature chain. E. coli derived.
Database link: P41159 -
ポジティブ･コントロール
- WB: Recombinant human Leptin.
製品の特性
-
製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ELISA |
Recombinant fragment
|
| WB |
Recombinant fragment
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 19 kDa.
Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
| ELISA |
Use a concentration of 0.25 - 1 µg/ml.
Human Leptin was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271278. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hLeptin protein. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 19 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
ELISA
Use a concentration of 0.25 - 1 µg/ml. Human Leptin was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271278. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hLeptin protein. |
ターゲット情報
-
機能
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
関連疾患
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
配列類似性
Belongs to the leptin family. -
細胞内局在
Secreted. - Information by UniProt
-
別名
- FLJ94114 antibody
- LEP antibody
- LEP_HUMAN antibody
see all
画像
-
Western blot - Biotin Anti-Leptin antibody (ab271278)
Lane 1: Marker.
Lane 2: Recombinant hLeptin at 250ng/lane.
Lane 3: Recombinant hLeptin at 125ng/lane.
Lane 4: Recombinant hLeptin at 62.5ng/lane.
Lane 5: Recombinant hLeptin at 31.25ng/lane.
Lane 6: Recombinant hLeptin at 15.625ng/lane.
Lane 7: Recombinant hLeptin at 7.8ng/lane.
Lane 8: Recombinant hLeptin at 3.9ng/lane.
Lane 9: Recombinant hLeptin at 1.95ng/lane.
Lane 10: Recombinant hLeptin at 0.975ng/lane.
Lane 11: Recombinant hLeptin at 0.4875ng/lane.
Lane 12: Recombinant hLeptin at 0.24ng/lane. -
ELISA - Biotin Anti-Leptin antibody (ab271278)Human Leptin was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271278. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hLeptin protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271278 は論文での使用が確認できていません。
