Biotin Anti-Klotho 抗体 (ab271241)

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Western blot - Biotin Anti-Klotho antibody (ab271241)
  • Sandwich ELISA - Biotin Anti-Klotho antibody (ab271241)

Key features and details

  • Biotin Rabbit polyclonal to Klotho
  • Suitable for: WB, Sandwich ELISA
  • Reacts with: Recombinant fragment
  • Conjugation: Biotin
  • Isotype: IgG

製品の概要

製品の特性

関連製品

    アプリケーション

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab271241 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    アプリケーション Species
    sELISA
    Recombinant fragment
    WB
    Recombinant fragment
    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 116 kDa.
    Sandwich ELISA
    Use at an assay dependent concentration.
    特記事項
    WB
    Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 116 kDa.
    Sandwich ELISA
    Use at an assay dependent concentration.

    ターゲット情報

    • 機能

      May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1.
      The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

    • 組織特異性

      Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.

    • 関連疾患

      Defects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement.

    • 配列類似性

      Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

    • ドメイン

      Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.

    • 翻訳後修飾

      N-glycosylated.

    • 細胞内局在

      Secreted and Cell membrane. Isoform 1 shedding leads to a soluble peptide.
    • Information by UniProt

    • 別名

      • Kl antibody
      • KLOT_HUMAN antibody
      • Klotho peptide antibody

    画像

    • Western blot - Biotin Anti-Klotho antibody (ab271241)
      Western blot - Biotin Anti-Klotho antibody (ab271241)

      Western blot analysis of varying levels of Recombinant human Klotho protein using ab271241 at 0.2 µg/ml.

      Used in conjunction with compatible secondary reagents the detection limit for recombinant Klotho is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

      Lane      ng/lane
      1           Marker
      2           250
      3           125
      4           62.5
      5           31.25
      6           15.625
      7           7.8
      8           3.9
      9           1.95
      10         0.975
      11         0.4875
      12         0.24

    • Sandwich ELISA - Biotin Anti-Klotho antibody (ab271241)
      Sandwich ELISA - Biotin Anti-Klotho antibody (ab271241)

      Human Klotho was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271241. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hKlotho protein.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab271241 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab271241 は論文での使用が確認できていません。

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