Biotin Anti-IL-16 抗体 (ab271199)
Key features and details
- Biotin Rabbit polyclonal to IL-16
- Suitable for: WB, ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-IL-16 antibody
IL-16 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to IL-16 -
由来種
Rabbit -
標識
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentWB Recombinant fragment
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免疫原
Recombinant fragment corresponding to Human IL-16 aa 1200-1350.
Database link: Q14005 -
ポジティブ･コントロール
- WB: Recombinant human IL-16 protein.
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ELISA |
Recombinant fragment
|
| WB |
Recombinant fragment
|
| All applications |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 142 kDa.
|
|
| ELISA |
Use a concentration of 0.25 - 1 µg/ml.
Human IL-16 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271199. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-16 protein. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 142 kDa. |
|
ELISA
Use a concentration of 0.25 - 1 µg/ml. Human IL-16 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271199. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-16 protein. |
ターゲット情報
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機能
Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. -
組織特異性
Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation. -
配列類似性
Contains 4 PDZ (DHR) domains. -
翻訳後修飾
Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16. -
細胞内局在
Cytoplasm; Cytoplasm. Nucleus and Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 3603 Human
- Omim: 603035 Human
- SwissProt: Q14005 Human
- Unigene: 459095 Human
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別名
- HGNC:5980 antibody
- HsT19289 antibody
- IL-16 antibody
see all
画像
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Western blot - Biotin Anti-IL-16 antibody (ab271199)
To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human IL-16, respectively.
Non-reducing conditions.
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ELISA - Biotin Anti-IL-16 antibody (ab271199)Human IL-16 was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271199.
This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant IL-16 protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271199 は論文での使用が確認できていません。
