Biotin Anti-IL-1 alpha 抗体 (ab271258)
Key features and details
- Biotin Rabbit polyclonal to IL-1 alpha
- Suitable for: WB, Sandwich ELISA
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-IL-1 alpha antibody
IL-1 alpha 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to IL-1 alpha -
由来種
Rabbit -
標識
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA MouseWB Mouse
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免疫原
Recombinant fragment corresponding to Mouse IL-1 alpha aa 100 to the C-terminus.
Database link: P01582
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| sELISA |
Mouse
|
| WB |
Mouse
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 31 kDa.
|
|
| Sandwich ELISA |
Use a concentration of 0.25 - 1 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 31 kDa. |
|
Sandwich ELISA
Use a concentration of 0.25 - 1 µg/ml. |
ターゲット情報
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機能
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. -
配列類似性
Belongs to the IL-1 family. -
ドメイン
The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function. -
細胞内局在
Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. - Information by UniProt
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参照データベース
- Entrez Gene: 16175 Mouse
- SwissProt: P01582 Mouse
- Unigene: 15534 Mouse
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別名
- BAF antibody
- FAF antibody
- Hematopoietin 1 antibody
see all
画像
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Western blot - Biotin Anti-IL-1 alpha antibody (ab271258)All lanes : Biotin Anti-IL-1 alpha antibody (ab271258) at 0.2 µg/ml
Lane 1 : Recombinant Mouse IL-1 alpha protein at 0.25 µg
Lane 2 : Recombinant Mouse IL-1 alpha protein at 0.125 µg
Lane 3 : Recombinant Mouse IL-1 alpha protein at 0.0625 µg
Lane 4 : Recombinant Mouse IL-1 alpha protein at 0.03125 µg
Lane 5 : Recombinant Mouse IL-1 alpha protein at 0.015625 µg
Lane 6 : Recombinant Mouse IL-1 alpha protein at 0.0078 µg
Lane 7 : Recombinant Mouse IL-1 alpha protein at 0.0039 µg
Lane 8 : Recombinant Mouse IL-1 alpha protein at 0.00195 µg
Lane 9 : Recombinant Mouse IL-1 alpha protein at 0.000975 µg
Lane 10 : Recombinant Mouse IL-1 alpha protein at 0.0004875 µg
Lane 11 : Recombinant Mouse IL-1 alpha protein at 0.00024 µg
Predicted band size: 31 kDa -
Sandwich ELISA - Biotin Anti-IL-1 alpha antibody (ab271258)Mouse IL-1 alpha was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271258. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mouse IL-1 alpha protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271258 は論文での使用が確認できていません。
