Biotin Anti-ICAM1 抗体 (ab271238)
Key features and details
- Biotin Rabbit polyclonal to ICAM1
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
-
製品名
Biotin Anti-ICAM1 antibody
ICAM1 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to ICAM1 -
由来種
Rabbit -
標識
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA Recombinant fragmentWB Recombinant fragment
-
免疫原
Recombinant fragment corresponding to Human ICAM1 aa 1-500.
Database link: P05362
製品の特性
-
製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| sELISA |
Recombinant fragment
|
| WB |
Recombinant fragment
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 57 kDa.
|
|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 57 kDa. |
|
Sandwich ELISA
Use at an assay dependent concentration. |
ターゲット情報
-
機能
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus. -
配列類似性
Belongs to the immunoglobulin superfamily. ICAM family.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
翻訳後修飾
Monoubiquitinated, which is promoted by MARCH9 and leads to endocytosis. -
細胞内局在
Membrane. - Information by UniProt
-
別名
- Antigen identified by monoclonal antibody BB2 antibody
- BB 2 antibody
- BB2 antibody
see all
画像
-
Western blot - Biotin Anti-ICAM1 antibody (ab271238)
Western blot analysis of varying levels of Recombinant human ICAM1 protein using ab271238 at 0.2 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant ICAM1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24 -
Sandwich ELISA - Biotin Anti-ICAM1 antibody (ab271238)Human ICAM-1 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271238. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Human ICAM-1 protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271238 は論文での使用が確認できていません。
