Biotin Anti-Granzyme B 抗体 [GZMB/3014] (ab271818)
Key features and details
- Biotin Mouse monoclonal [GZMB/3014] to Granzyme B
- Suitable for: IHC-P, Protein Array
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2b
製品の概要
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製品名
Biotin Anti-Granzyme B antibody [GZMB/3014]
Granzyme B 一次抗体 製品一覧 -
製品の詳細
Biotin Mouse monoclonal [GZMB/3014] to Granzyme B -
由来種
Mouse -
標識
Biotin -
Tested Applications & Species
Application Species IHC-P Human -
免疫原
Recombinant fragment within Mouse Granzyme B aa 73-187. The exact sequence is proprietary.
Database link: P04187 -
ポジティブ・コントロール
- IHC-P: Human spleen tissue.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
GZMB/3014 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
アプリケーション | Species |
---|---|
IHC-P |
Human
|
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
Protein Array |
Use at an assay dependent concentration.
|
特記事項 |
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IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Protein Array
Use at an assay dependent concentration. |
ターゲット情報
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機能
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
配列類似性
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
細胞内局在
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
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参照データベース
- Entrez Gene: 3002 Human
- Omim: 123910 Human
- SwissProt: P10144 Human
- Unigene: 1051 Human
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別名
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Granzyme B antibody [GZMB/3014] (ab271818)
Formalin-fixed, paraffin-embedded human spleen tissue stained for Granzyme B using Biotinylated ab271818 at 4 µg/ml in
immunohistochemical analysis
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Granzyme B antibody [GZMB/3014] (ab271818)
Formalin-fixed, paraffin-embedded human spleen tissue stained for Granzyme B using Biotinylated ab271818 at 4 µg/ml in immunohistochemical analysis
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Analysis of Protein Array containing >19,000 full-length human proteins using Granzyme B ab271818. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab271818 は論文での使用が確認できていません。