Biotin Anti-Fluorescein 抗体 [BT.6A4] (ab6215)

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Key features and details

  • Biotin Mouse monoclonal [BT.6A4] to Fluorescein
  • Suitable for: WB, ELISA, IHC-P, IHC-Fr, In situ hybridization
  • Reacts with: Species independent
  • Conjugation: Biotin
  • Isotype: IgM

製品の概要

  • 製品名

    Biotin Anti-Fluorescein antibody [BT.6A4]
    Fluorescein 一次抗体 製品一覧

  • 製品の詳細

    Biotin Mouse monoclonal [BT.6A4] to Fluorescein

  • 由来種

    Mouse

  • 標識

    Biotin

  • 特異性

    The antibody reacts with free and bound fluorescein.

  • アプリケーション

    適用あり: WB, ELISA, IHC-P, IHC-Fr, In situ hybridizationmore details

  • 種交差性

    交差種: Species independent

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.40
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...

  • 特記事項（精製）

    Purified anti-fluorescein biotin conjugate.

  • ポリ/モノ

    モノクローナル

  • クローン名

    BT.6A4

  • ミエローマ

    unknown

  • アイソタイプ

    IgM

  • 軽鎖の種類

    kappa

  • 研究分野

関連製品

    アプリケーション

    Our Abpromise guarantee covers the use of ab6215 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB 1/500 - 1/5000.
    ELISA 1/1000 - 1/10000.
    IHC-P 1/200 - 1/2000.
    IHC-Fr 1/200 - 1/2000.
    In situ hybridization Use at an assay dependent dilution.

    ターゲット情報

    • 関連性

      Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.

    • 別名

      • FI antibody
      • FITC antibody
      • fluorescein isothyocianate antibody
      • fluorescyl antibody

    参考文献 (1)

    ab6215 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6215 は 1 報の論文で使用されています。

    • Mukaiyama M  et al. Non-electrophilic TRPA1 agonists, menthol, carvacrol and clotrimazole, open epithelial tight junctions via TRPA1 activation. J Biochem N/A:N/A (2020). PubMed: 32428205

    レビューと Q&A

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    1-2 of 2 Abreviews or Q&A

    Question

    We typically use an anti-fluoroscein HRP antibody for southern blotting and it seems that the anti-fluoroscein biotinylated antibody would be the most comparable. My question is what detection reagents do you suggest to be used with the anti-fluoroscein biotinylated antibody that you sell??

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    Answer

    You could detect the biotinylated antibody directly or use a secondary to the fluorescein antibody.

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    Question

    Has your anti fluorescein antibody be tested in Flow cytometry? I am thinking about labeling cells with CFSE which labels the cytoplasm. Over time the signal weakens and I was hoping to increase the signal by using an anti fluorescein mAb after I permiablize the cells.

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    Answer

    This antibody has not been tested in flow cytometry. However, in theory it should work in your application.

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