Biotin Anti-Fluorescein 抗体 (ab6655)
Key features and details
- Biotin Goat polyclonal to Fluorescein
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, In situ hybridization, WB
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-Fluorescein antibody
Fluorescein 一次抗体 製品一覧 -
製品の詳細
Biotin Goat polyclonal to Fluorescein -
由来種
Goat -
標識
Biotin -
アプリケーション
適用あり: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, In situ hybridization, WBmore details -
種交差性
交差種: Species independent -
免疫原
Fluorescein conjugated to Goat IgG
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特記事項
Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per goat IgG molecule.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab6655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Dot blot | Use at an assay dependent concentration. | |
| ELISA | 1/10000 - 1/50000. | |
| IHC-P | 1/1000 - 1/5000. | |
| IHC-Fr | 1/1000 - 1/5000. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| In situ hybridization | Use at an assay dependent concentration. | |
| WB | 1/2000 - 1/10000. |
ターゲット情報
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関連性
Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters. -
別名
- FI antibody
- FITC antibody
- fluorescein isothyocianate antibody
- fluorescyl antibody
画像
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Western blot - Biotin Anti-Fluorescein antibody (ab6655)Biotin Anti-Fluorescein antibody (ab6655) at 1/1000 dilution + BSA conjugated Fluorescein.
Secondary
Peroxidase conjugated Streptavidin secondary antibody at 1/40000 dilution -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fluorescein antibody (ab6655)Cropped image from Long et al, Nature Communications, 7, 10354, Fig. 7a.; doi: 10.1038/ncomms10354 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immunohistochemical analysis of chicken embryo neuroepithelium labelling fluorescein-tagged morpholinos with ab6655. Samples were fixed in 4% paraformaldehyde at 4°C for 1 hour.
プロトコール
参考文献 (2)
ab6655 は 2 報の論文で使用されています。
- He B et al. Vascular targeting of LIGHT normalizes blood vessels in primary brain cancer and induces intratumoural high endothelial venules. J Pathol 245:209-221 (2018). PubMed: 29603739
- Long K et al. Integrin signalling regulates the expansion of neuroepithelial progenitors and neurogenesis via Wnt7a and Decorin. Nat Commun 7:10354 (2016). IHC-Fr, IHC-FrFl . PubMed: 26838601
