Application
ELISA
Sample
Escherichia coli Purified protein (M13 phage reacted to Fc protein)
Specification
M13 phage reacted to Fc protein
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 4°C
Type
Indirect
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 4000
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: StreptAvidin
Host species: Streptomyces
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Streptomyces
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
We found that it worked well for phage-ELISA application.
M13 phage displaying anti Fc antibody was applied to ELISA plate coated purified Fc protein or BSA (negative control). The binding of M13 phage to Fc was evaluated by adding anti-M13 fd F1 antibody (biotin) followed by StreptAvidin HRP.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 27 2018