Biotin Anti-Filaggrin 抗体 [FLG/1563] (ab271816)
Key features and details
- Biotin Mouse monoclonal [FLG/1563] to Filaggrin
- Suitable for: IHC-P, Protein Array
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG2b
製品の概要
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製品名
Biotin Anti-Filaggrin antibody [FLG/1563]
Filaggrin 一次抗体 製品一覧 -
製品の詳細
Biotin Mouse monoclonal [FLG/1563] to Filaggrin -
由来種
Mouse -
標識
Biotin -
Tested Applications & Species
Application Species IHC-P Human -
免疫原
Recombinant fragment within Human Filaggrin aa 998-1104. The exact sequence is proprietary.
Database link: P20930 -
ポジティブ・コントロール
- IHC-P: Human skin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from Bioreactor Concentrate. -
ポリ/モノ
モノクローナル -
クローン名
FLG/1563 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271816 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
アプリケーション | Species |
---|---|
IHC-P |
Human
|
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
Protein Array |
Use at an assay dependent concentration.
|
特記事項 |
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IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Protein Array
Use at an assay dependent concentration. |
ターゲット情報
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機能
Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis. -
組織特異性
Keratohyalin granules. -
関連疾患
Ichthyosis vulgaris
Dermatitis atopic 2 -
配列類似性
Belongs to the S100-fused protein family.
Contains 2 EF-hand domains.
Contains 23 filaggrin repeats. -
翻訳後修飾
Filaggrin is initially synthesized as a large, insoluble, highly phosphorylated precursor containing many tandem copies of 324 AA, which are not separated by large linker sequences. During terminal differentiation it is dephosphorylated and proteolytically cleaved. The N-terminal of the mature protein is heterogeneous, and is blocked by the formation of pyroglutamate.
Undergoes deimination of some arginine residues (citrullination). - Information by UniProt
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参照データベース
- Entrez Gene: 2312 Human
- Omim: 135940 Human
- SwissProt: P20930 Human
- Unigene: 654510 Human
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別名
- ATOD2 antibody
- Epidermal filaggrin antibody
- FILA_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Filaggrin antibody [FLG/1563] (ab271816)
Formalin-fixed, paraffin-embedded human skin for filaggrin using ab271816 at 4µg/mL in immunohistochemical analysis.
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Analysis of Protein Array containing more than 19,000 full-length human proteins using Filaggrin ab271816.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271816 は論文での使用が確認できていません。