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    biotin-fibronectin-antibody-ab6584.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Extracellular Matrix
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Biotin Anti-Fibronectin 抗体 (ab6584)

  • Datasheet
  • SDS
Submit a review Q&A (10)References (40)

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Western blot - Biotin Anti-Fibronectin antibody (ab6584)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (ab6584)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (ab6584)

Key features and details

  • Biotin Rabbit polyclonal to Fibronectin
  • Suitable for: IHC-P, WB
  • Reacts with: Human, Recombinant fragment
  • Conjugation: Biotin
  • Isotype: IgG

こちらの製品もご検討ください

標識
Product image
Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
ELISA
Product image
Human Fibronectin ELISA Kit (ab219046)
一次抗体
Product image
Anti-MMP9 antibody [EPR22140-154] (ab228402)

関連製品

製品の概要

  • 製品名

    Biotin Anti-Fibronectin antibody
    Fibronectin 一次抗体 製品一覧
  • 製品の詳細

    Biotin Rabbit polyclonal to Fibronectin
  • 由来種

    Rabbit
  • 標識

    Biotin
  • 特異性

    negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Laminin. Non-specific cross reaction of anti-Fibronectin antibodies with other human serum proteins or non-Fibronectin extracellular matrix proteins is negligible.
  • アプリケーション

    適用あり: IHC-P, WBmore details
  • 種交差性

    交差種: Human, Recombinant fragment
    交差が予測される動物種: Mammals
  • 免疫原

    Full length native protein (purified) corresponding to Human Fibronectin. Purified from human plasma by binding to a denatured gelatin column followed by elution with high concentrations of arginine. The eluted material was further purified by gel filtration.
    Database link: P02751

  • 特記事項


    This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other extracellular matrix proteins was detected by ELISA against purified standards.
  • 一次抗体 備考

    This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cardiovascular
    • Angiogenesis
    • Adhesion / ECM
    • Extracellular Matrix
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Fibronectin
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Neural Stem Cells
    • Extracellular
    • Stem Cells
    • Mesenchymal Stem Cells
    • Osteogenesis
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Developmental Biology
    • Lineage specification
    • Ectoderm

関連製品

  • Isotype control

    • Biotin Rabbit IgG - Isotype Control (ab200208)
  • Recombinant Protein

    • Recombinant Human Fibronectin type III EDB protein (ab209886)
  • Related Products

    • Native Mouse Fibronectin protein (ab92784)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6584の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/50 - 1/200.
WB
1/5000 - 1/10000.
特記事項
IHC-P
1/50 - 1/200.
WB
1/5000 - 1/10000.

ターゲット情報

  • 機能

    Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
    Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
  • 組織特異性

    Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.
  • 関連疾患

    Glomerulopathy with fibronectin deposits 2
  • 配列類似性

    Contains 12 fibronectin type-I domains.
    Contains 2 fibronectin type-II domains.
    Contains 16 fibronectin type-III domains.
  • 発生段階

    Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
  • 翻訳後修飾

    Sulfated.
    It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated.
    Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers).
    Phosphorylated by FAM20C in the extracellular medium.
    Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
  • 細胞内局在

    Secreted, extracellular space, extracellular matrix.
  • Target information above from: UniProt accession P02751 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 2335 Human
    • Omim: 135600 Human
    • SwissProt: P02751 Human
    • Unigene: 203717 Human
    • 別名

      • CIG antibody
      • Cold insoluble globulin antibody
      • Cold-insoluble globulin antibody
      • DKFZp686F10164 antibody
      • DKFZp686H0342 antibody
      • DKFZp686I1370 antibody
      • DKFZp686O13149 antibody
      • ED B antibody
      • Fibronectin 1 antibody
      • FINC antibody
      • FINC_HUMAN antibody
      • FN antibody
      • FN1 antibody
      • FNZ antibody
      • GFND antibody
      • GFND2 antibody
      • LETS antibody
      • Migration stimulating factor antibody
      • MSF antibody
      • Ugl-Y3 antibody
      see all

    画像

    • Western blot - Biotin Anti-Fibronectin antibody (ab6584)
      Western blot - Biotin Anti-Fibronectin antibody (ab6584)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (ab6584)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (ab6584)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue sections at pH 6.0, labelling Fibronectin with ab6584 at a concentration of 10 µg/mL for 1 hour at room temperature. The secondary used was a rabbit peroxidase conjugate at a 1/10,000 dilution, incubated for 45 minutes at room temperature. Fibronectin is shown as a brown hue against a purple counterstain labelling nuclear DNA. The negative control on right (B) is without the primary antibody. Magnifications are as shown.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (ab6584)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Fibronectin antibody (ab6584)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded human kidney tissue sections at pH 9.0, labelling Fibronectin with ab6584 at a concentration of 10 µg/mL for 1 hour at room temperature. The secondary used was a rabbit peroxidase conjugate at a 1/10,000 dilution, incubated for 45 minutes at room temperature. Fibronectin is shown as a brown hue against a purple counterstain labelling nuclear DNA. The negative control on right (B) is without the primary antibody. Magnifications are as shown.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (40)

    ab6584 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6584 は 40 報の論文で使用されています。

    • Bonvallet PP  et al. Biophysical Characterization of a Novel Tri-Layer Placental Allograft Membrane. Adv Wound Care (New Rochelle) N/A:N/A (2021). PubMed: 33975444
    • Yemanyi F  et al. Dexamethasone and Glucocorticoid-Induced Matrix Temporally Modulate Key Integrins, Caveolins, Contractility, and Stiffness in Human Trabecular Meshwork Cells. Invest Ophthalmol Vis Sci 61:16 (2020). PubMed: 33170205
    • Moore MC  et al. Biological characterization of dehydrated amniotic membrane allograft: Mechanisms of action and implications for wound care. J Biomed Mater Res B Appl Biomater N/A:N/A (2020). PubMed: 32458591
    • Gay D  et al. Phagocytosis of Wnt inhibitor SFRP4 by late wound macrophages drives chronic Wnt activity for fibrotic skin healing. Sci Adv 6:eaay3704 (2020). PubMed: 32219160
    • Alcalde-Estévez E  et al. Endothelin-1 induces cellular senescence and fibrosis in cultured myoblasts. A potential mechanism of aging-related sarcopenia. Aging (Albany NY) 12:11200-11223 (2020). PubMed: 32572011
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 10 Abreviews or Q&A

    Question

    I am looking for a way to identify human/bovine fibronectin in a confocal/fluorescent micrscope. Would this product require a secondary FITC antibody in order to be visualised? Is there a better choice and if so could you direct me there!

    Read More

    Abcam community

    Verified customer

    Asked on Nov 17 2005

    Answer

    Thank you for your enquiry about our fibronectin antibodies. The antibody ab6584 is biotin conjugated and has been successfully used in IF (ICC) by a user of the antibody who left a review which can be accessed via the online datasheet. The reviewer did not leave many details about the cells or protocol used so you may wish to contact him by clicking on his name on the sheet. As the antibody is biotin conjugated you will need to add an extra-avidin fluorescence conjugate (or avidin), so that the avidin part will bind to the biotin molecules (so you won't need a secondary antibody per se). You can purchase extra-avidin FITC for example and incubate it 1-3 hours with the cells; we currently have no information about the use of ab6584 in IF on sections. We have a similar anti fibronectin antibody unconjugated, ab299, but it has not been tested in IF/ICC or IHC, therefore we do not know if it will work in these applications. Please do not hesitate to contact me if you would like some protocol help with our antibodies or any further information,

    Read More

    Abcam Scientific Support

    Answered on Nov 18 2005

    Question

    What is the sensitivity of this antibody in an indirect ELISA - that is, what is the range that can be detected (minimum - maximum)?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 05 2005

    Answer

    For testing 1ug of antigen, the minimum of antibody used is 1:8000. The maximum is 1:4000. We don't have other data to show the sensitivity. If you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    Answered on Aug 05 2005

    Question

    What is the sensitivity of this antibody in an indirect ELISA?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 05 2005

    Answer

    1:4000 to 1:8000 dilution can detect 1.0ug of Fibronectin. If you have any additional questions, please contact us again.

    Read More

    Abcam Scientific Support

    Answered on Aug 05 2005

    Question

    BATCH NUMBER 54185 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM I am getting strong cytoplasmic staining in plasma cells. Should this antibody stain plasma cells? I am also getting staining in the ecm and in fibroblasts as expected. SAMPLE Human mucosa PRIMARY ANTIBODY anti-fibronectin (biotinylated) DETECTION METHOD ABC for 1hour at room temperature POSITIVE AND NEGATIVE CONTROLS USED Positive: salivary gland adenoid cystic carcinoma Negative: omitted primary antibody used an irrelevant antibody (broad range cytokeratin) ANTIBODY STORAGE CONDITIONS Aliquotted and stored at -80degC FIXATION OF SAMPLE 10% formol saline ANTIGEN RETRIEVAL Heat BLOCKING CONDITIONS normal rabbit serum for 10 minutes SECONDARY ANTIBODY nil HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes

    Read More

    Abcam community

    Verified customer

    Asked on Jun 10 2005

    Answer

    I have not found reports of staining of fibronectin in the cytoplasm of plasma cells, however it is known that plasma cells adhere to fibronectin (the fibronectin inducing not only the adherence and spread of plasma cells but also their migration). I therefore think that the staining you are observing is not specific (especially using normal rabbit serum as blocking agent) and would like to suggest the following changes to your protocol: -what type of IHC are you testing, is it paraffin embedded tissue? If so you need to consider antigen retrieval methods (heat mediated, enzymatic) - you did not mention quenching the endogenous peroxidases prior to start the staining protocol, please make sure you do as the DAB step will give high background if you have not incubated the tissue in H2O2 at the beginning of the protocol to quench endogenous peroxidase -block the tissue in normal goat/horse serum (not rabbit, as the primary antibody will bind to the serum, giving high background/non specific staining) -try decreasing the concentration of the primary antibody and changing the incubation time, temperature and the presence of triton in the dilution buffer -Incubation in ABC could be reduced to 1/2h I hope these recommendations will help you, please do not hesitate to contact me again if you still have problems,

    Read More

    Abcam Scientific Support

    Answered on Jun 14 2005

    Question

    I have just purchased this antibody (ab6584, batch no 54185). My problem is that today, when I opened the vial in order to aliquot and freeze, I realised that there's hardly any antibody in the vial. I calculated about 40 microliters. Is this how much the vial should contain? Being a student (I am buying antibodies for research related to an M Phil project) I cannot afford to but more vials. Could you suggest a solution? (e.g. would amplifying the signal work?)

    Read More

    Abcam community

    Verified customer

    Asked on Jul 09 2004

    Answer

    Thank you for your enquiry. We would like to confirm that the concentration is 2.5mg/mL, so 40uL will give 100ug of antibody.

    Read More

    Abcam Scientific Support

    Answered on Jul 12 2004

    Question

    Is antigen retrieval required for this antibody (IHC-paraffin)? If so, what method do you recommend?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 13 2003

    Answer

    This antibody does not require antigen retrieval but it may increase staining. We normally recommend the pressure cooker method of antigen retrieval.

    Read More

    Asdf Edo

    Abcam Scientific Support

    Answered on Oct 13 2003

    Question

    For westerns, what is the expected MW of Fibronectin that this antibody detects?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 10 2003

    Answer

    450 kDa non-reduced 225 kDa fully reduced

    Read More

    Abcam Scientific Support

    Answered on Jun 11 2003

    Question

    Hello, Is this antibody suitable for detecting fibroblast in mouse tissue ( since there is no suitable antibody available for fibroblasts to do immunohistochemistry ?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 08 2002

    Answer

    This antibody will cross-ract with mouse fibronectin using formalin fixed paraffin embedded tissues.

    Read More

    Abcam Scientific Support

    Answered on Aug 09 2002

    Question

    Hello: Is this antibody available in unconjugated form. I need a reagent for immunofluorescence quite urgently.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 21 2002

    Answer

    Yes, this antibody is available in an unconjugated form, ab299

    Read More

    Abcam Scientific Support

    Answered on Jun 24 2002

    Question

    Will this antibody recognize porcine fibronectin? Thanks, Liz Kaback

    Read More

    Abcam community

    Verified customer

    Asked on Jul 02 2001

    Answer

    The extracellular matrix proteins are well conserved, particularly between mammals, so human will cross-react with pig.

    Read More

    Neil Hayward

    Abcam Scientific Support

    Answered on Jul 03 2001

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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