Biotin Anti-CXCL5 抗体 (ab271281)
Key features and details
- Biotin Rabbit polyclonal to CXCL5
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-CXCL5 antibody
CXCL5 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to CXCL5 -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: WB, Sandwich ELISAmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human CXCL5 aa 1 to the C-terminus. E. coli derived.
Database link: P42830 -
ポジティブ・コントロール
- WB: Recombinant human CXCL5.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab271281の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa.
Used in conjunction with compatible secondary reagents the detection limit for recombinant human CXCL5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
Sandwich ELISA |
Use at an assay dependent concentration.
|
特記事項 |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 12 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant human CXCL5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
細胞内局在
Secreted. - Information by UniProt
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別名
- AMCFII antibody
- C-X-C motif chemokine 5 antibody
- C-X-C motif chemokine ligand 5 antibody
see all
画像
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Lane 1: Marker.
Lane 2: Recombinant hCXCL5 at 250ng/lane.
Lane 3: Recombinant hCXCL5 at 125ng/lane.
Lane 4: Recombinant hCXCL5 at 62.5ng/lane.
Lane 5: Recombinant hCXCL5 at 31.25ng/lane.
Lane 6: Recombinant hCXCL5 at 15.625ng/lane.
Lane 7: Recombinant hCXCL5 at 7.8ng/lane.
Lane 8: Recombinant hCXCL5 at 3.9ng/lane.
Lane 9: Recombinant hCXCL5 at 1.95ng/lane.
Lane 10: Recombinant hCXCL5 at 0.975ng/lane.
Lane 11: Recombinant hCXCL5 at 0.4875ng/lane.
Lane 12: Recombinant hCXCL5 at 0.24ng/lane. -
Human ENA-78 (CXCL5) was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271281. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hENA-78 protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab271281 は論文での使用が確認できていません。