Biotin Anti-CXCL16 抗体 (ab271222)
Key features and details
- Biotin Rabbit polyclonal to CXCL16
- Suitable for: WB, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-CXCL16 antibody
CXCL16 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to CXCL16 -
由来種
Rabbit -
標識
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species sELISA HumanWB Recombinant fragment
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免疫原
Recombinant fragment corresponding to Human CXCL16 aa 1-150.
Database link: Q9H2A7
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271222 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| sELISA |
Human
|
| WB |
Recombinant fragment
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 28 kDa.
|
|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 28 kDa. |
|
Sandwich ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. -
組織特異性
Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
Glycosylated. -
細胞内局在
Cell membrane. Secreted. Also exists as a soluble form. - Information by UniProt
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参照データベース
- Entrez Gene: 58191 Human
- Omim: 605398 Human
- SwissProt: Q9H2A7 Human
- Unigene: 513885 Human
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別名
- C-X-C motif chemokine 16 antibody
- Chemokine (C X C motif) ligand 16 antibody
- Chemokine, CXC motif, ligand 16 antibody
see all
画像
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Western blot - Biotin Anti-CXCL16 antibody (ab271222)
To detect hCXCL16 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant hCXCL16 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane 1: Marker
Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant human CXCL16, respectively.
Non-reducing conditions.
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Sandwich ELISA - Biotin Anti-CXCL16 antibody (ab271222)Human hCXCL16 was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271222.
This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hCXCL16 protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271222 は論文での使用が確認できていません。
