Biotin Anti-Collagen IV 抗体 (ab6581)

Reviews (1)Q&A (6)References (7)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Collagen IV antibody (ab6581)

    Key features and details

    • Biotin Rabbit polyclonal to Collagen IV
    • Suitable for: ELISA, WB, IP, IHC-P
    • Reacts with: Cow, Human
    • Conjugation: Biotin
    • Isotype: IgG

    製品の概要

    • 製品名

      Biotin Anti-Collagen IV antibody
      Collagen IV 一次抗体 製品一覧

    • 製品の詳細

      Biotin Rabbit polyclonal to Collagen IV

    • 由来種

      Rabbit

    • 標識

      Biotin

    • 特異性

      negligible cross-reactivity with Type I, II, III, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.

    • アプリケーション

      適用あり: ELISA, WB, IP, IHC-Pmore details

    • 種交差性

      交差種: Cow, Human
      交差が予測される動物種: Mammals

    • 免疫原

      Full length native protein (purified) corresponding to Collagen IV. Collagen Type IV from human and bovine placenta.

    • 特記事項

      At least 11 genetically distinct gene products are collectively referred to as 'collagen types' or other proteins and proteoglycans of the extracellular matrix. In humans, collagens are composed of about 20 unique protein chains which under go various types of post-translational modifications and are ultimately assembled into a triple helix. This results in great diversity between collagen types. Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.


      These antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      Preservative: 0.01% Sodium azide
      Constituents: 0.44% Sodium chloride, 1% BSA, 4.77% Sodium borate, 0.146% EDTA
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities.

    • 一次抗体 備考

      These antibodies are well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab6581の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ELISA (1)
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 161 kDa.

    Not recommended for use under denaturing conditions.
    IP
    Use at an assay dependent concentration.
    IHC-P
    1/1000 - 1/5000.
    特記事項
    ELISA
    Use at an assay dependent concentration.
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 161 kDa.

    Not recommended for use under denaturing conditions.
    IP
    Use at an assay dependent concentration.
    IHC-P
    1/1000 - 1/5000.

    ターゲット情報

    • 機能

      Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
      Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.

    • 組織特異性

      Highly expressed in placenta.

    • 関連疾患

      Defects in COL4A1 are a cause of brain small vessel disease with hemorrhage (BSVDH) [MIM:607595]. Brain small vessel diseases underlie 20 to 30 percent of ischemic strokes and a larger proportion of intracerebral hemorrhages. Inheritance is autosomal dominant.
      Defects in COL4A1 are the cause of hereditary angiopathy with nephropathy aneurysms and muscle cramps (HANAC) [MIM:611773]. The clinical renal manifestations include hematuria and bilateral large cysts. Histologic analysis revealed complex basement membrane defects in kidney and skin. The systemic angiopathy appears to affect both small vessels and large arteries.
      Defects in COL4A1 are a cause of porencephaly familial (PCEPH) [MIM:175780]. Porencephaly is a term used for any cavitation or cerebrospinal fluid-filled cyst in the brain. Porencephaly type 1 is usually unilateral and results from focal destructive lesions such as fetal vascular occlusion or birth trauma. Type 2, or schizencephalic porencephaly, is usually symmetric and represents a primary defect or arrest in the development of the cerebral ventricles.

    • 配列類似性

      Belongs to the type IV collagen family.
      Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.

    • ドメイン

      Alpha chains of type IV collagen have a non-collagenous domain (NC1) at their C-terminus, frequent interruptions of the G-X-Y repeats in the long central triple-helical domain (which may cause flexibility in the triple helix), and a short N-terminal triple-helical 7S domain.

    • 翻訳後修飾

      Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.
      Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
      Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.
      The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.
      Proteolytic processing produces the C-terminal NC1 peptide, arresten.

    • 細胞内局在

      Secreted > extracellular space > extracellular matrix > basement membrane.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • Arresten antibody
      • BSVD antibody
      • CO4A1_HUMAN antibody
      • COL4A1 antibody
      • COL4A1 NC1 domain antibody
      • COL4A2 antibody
      • COL4A3 antibody
      • COL4A4 antibody
      • COL4A5 antibody
      • collagen alpha-1(IV) chain antibody
      • Collagen IV Alpha 1 Polypeptide antibody
      • Collagen IV Alpha 2 Polypeptide antibody
      • Collagen Of Basement Membrane Alpha 1 Chain antibody
      • Collagen Of Basement Membrane Alpha 2 Chain antibody
      • Collagen Type IV Alpha 1 antibody
      • collagen type IV alpha 1 chain antibody
      • Collagen Type IV Alpha 2 antibody
      • Collagen Type IV Alpha 3 antibody
      • Collagen Type IV Alpha 4 antibody
      • Collagen Type IV Alpha 5 antibody
      • RATOR antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Collagen IV antibody (ab6581)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Collagen IV antibody (ab6581)

      Immunohistochemical analysis of formalin-fixed paraffin-embedded human tissue sections, labelling Collagen IV with ab6581 at a concentration of 10 µg/mL for 1 hour at room temperature. The left panel is human kidney sections with the right panel being human liver sections. Antigen retrival was performed with 0.01 M sodium citrate buffer at pH 6.0 at 99°C for 20 mins. The secondary used was a rabbit peroxidase secondary antibody at a 1/10,000 dilution incubated for 45 mins at room temperature. Counterstaining against nuclear DNA was hematoxylin.

    参考文献 (7)

    ab6581 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6581 は 7 報の論文で使用されています。

    • Kargar-Abarghouei E  et al. Characterization, recellularization, and transplantation of rat decellularized testis scaffold with bone marrow-derived mesenchymal stem cells. Stem Cell Res Ther 9:324 (2018). PubMed: 30463594
    • Hassanpour A  et al. Decellularized human ovarian scaffold based on a sodium lauryl ester sulfate (SLES)-treated protocol, as a natural three-dimensional scaffold for construction of bioengineered ovaries. Stem Cell Res Ther 9:252 (2018). PubMed: 30257706
    • Buno KP  et al. In Vitro Multitissue Interface Model Supports Rapid Vasculogenesis and Mechanistic Study of Vascularization across Tissue Compartments. ACS Appl Mater Interfaces 8:21848-60 (2016). PubMed: 27136321
    • Zhou J  et al. Qianliening capsules influence the apoptosis of benign prostatic hyperplasia epithelial-1 cells by regulating the extracellular matrix. Mol Med Rep 11:3734-40 (2015). PubMed: 25592406
    • Whittington CF  et al. Collagen-polymer guidance of vessel network formation and stabilization by endothelial colony forming cells in vitro. Macromol Biosci 13:1135-49 (2013). IHC . PubMed: 23832790
    • Vandenbroucke RE  et al. Matrix metalloprotease 8-dependent extracellular matrix cleavage at the blood-CSF barrier contributes to lethality during systemic inflammatory diseases. J Neurosci 32:9805-16 (2012). IHC ; Mouse . PubMed: 22815495
    • Thomas SN  et al. Impaired humoral immunity and tolerance in K14-VEGFR-3-Ig mice that lack dermal lymphatic drainage. J Immunol 189:2181-90 (2012). PubMed: 22844119

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    1-7 of 7 Abreviews or Q&A

    ELISA abreview for Anti-Collagen IV antibody (Biotin)

     Average
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Purified protein (collagen IV)
    Specification
    collagen IV
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
    Type
    Sandwich (Detection)
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    Abcam user community

    Verified customer

    投稿 Aug 16 2017

    Question

    Is that under denaturing conditions? 400KDA is a very high molecular weight normally it is hard to get it into tris glycine electrophoresis gels unless we reduce it first with BME or DTT reducing agents. Thanks,

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    Answer

    Thank you for your enquiry. Yes, 400kDa is very large. May I advise you use lower percentage gels, i.e. 5% or 4-8% gradient. I know it is not easy. We recommend non-denaturing, non-dissociating SDS-PAGE. I have been informed that there is considerable conflicting data in the literature when Collagens are reduced. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    What is the molecular weight of this protein as detected by Western blot?

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    Answer

    Thank you for your enquiry. The native collagen IV is about 400kDa following detection by western blot. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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    Question

    Customer would like to know the concentration, if possible, of this ab. Also, she received 10 ul in her vial and is concerned b/c that seems low.

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    Answer

    The concentration of the lot that you received is 2.15 mg/ml and you should have received 47 ul in the vial. I apologize for this shortage and will have another vial sent to you free of charge. Can you send me your shipping address including phone number? Also, please tell me what the original Abcam order number was.

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    Question

    AB6581-100 Please let me know the crossreactivity with rat? Best regards, Jonas Hink

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    Answer

    This antibody has been tested on various mammalian tissue (although not rat) and shown to work. Given the conservation of the collagen famiily we predict that this antibody will work well on rats.

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    Question

    Please inform me the concentration of immunoglobulin and the volume of liquid.

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    Answer

    The protein concnetration is 1mg/ml and the vial size is 0.1ml.

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    Question

    Hello, #AB6581-100(Anti collagen type IV) Could you please let us know the crossreactivity with mouse? I look forward to receiving your early reply. Best regards, Maki Kobayashi

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    Answer

    The anti-collagen antibody should cross-react with mouse.

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