Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Lung)
Permeabilization
No
Specification
Lung
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 1% BSA in TBS
Dilution
1/250
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
- Endogenous peroxidases blocked with 3% H2O2 in ethanol
- Biotin conjugated with Elite ABC staining kit
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Simon Cleary
Verified customer
投稿 May 29 2015