Biotin Anti-CD4 抗体 [RPA-T4] (ab272331)
![Flow Cytometry - Biotin Anti-CD4 antibody [RPA-T4] (ab272331)](https://www.abcam.co.jp/ps/products/272/ab272331/Images/ab272331-388007-anti-human-cd4-flow-cytometry.jpg "Flow Cytometry - Biotin Anti-CD4 antibody [RPA-T4] (ab272331)")
Key features and details
- Biotin Mouse monoclonal [RPA-T4] to CD4
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG1
製品の概要
-
製品名
Biotin Anti-CD4 antibody [RPA-T4]
CD4 一次抗体 製品一覧 -
製品の詳細
Biotin Mouse monoclonal [RPA-T4] to CD4 -
由来種
Mouse -
標識
Biotin -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee
-
免疫原
The details of the immunogen for this antibody are not available.
-
ポジティブ･コントロール
- Flow Cyt: Human peripheral blood lymphocytes.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.12% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Purified from TCS. Purified antibody was conjugated under optimal conditions, with unreacted biotin removed from the preparation. -
ポリ/モノ
モノクローナル -
クローン名
RPA-T4 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
Alternative Versions
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272331 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
配列類似性
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
細胞内局在
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
-
参照データベース
- Entrez Gene: 450124 Chimpanzee
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P16004 Chimpanzee
- SwissProt: P01730 Human
- Unigene: 631659 Human
-
別名
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
画像
-
Flow Cytometry - Biotin Anti-CD4 antibody [RPA-T4] (ab272331)
Flow cytometric analysis of human peripheral blood lymphocytes labeling CD4 using 0.25 µg of ab272331 (solid line) or 0.25 µg Biotin Mouse IgG1 isotype control (dashed line), followed by Streptavidin PE.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab272331 は論文での使用が確認できていません。