Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain,lateral ventricle)
Specification
brain,lateral ventricle
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Tris Buffered Saline- 0.3% Triton X
Permeabilization
Yes - 4N HCl
Blocking step
NDS as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: Tris Buffered Saline and 0,3% Triton X
Secondary antibody
Name
Non-Abcam antibody was used: anti sheep-Vector/Szabo
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Typical Brdu positive DAB-staining of mouse brain, subventricular zone of lateral ventricle 10X magnification.In the right upper part, a magnification of these Brdu positive cells that are going to migrate in the rostra migratory stream (RSM) to the olfactory bulb and differentiate into neurons and glia.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. stefano gaburro
Verified customer
投稿 Nov 01 2007