Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Cell (free floating Brain slices)
Specification
free floating Brain slices
Fixative
Paraformaldehyde
Antigen retrieval step
None
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10
Other product details
Dilution
1/2000
Incubation time
24 hour(s) and 0 minute(s)
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
The image depicts the subventricular zone.
A strpetavidin-HRP complex and DAB were used for detection.
Immunohistochemistry was performed on free floating 40 µm vibratome sections obtained from paraformaldehyde fixed brains
A strpetavidin-HRP complex and DAB were used for detection.
Immunohistochemistry was performed on free floating 40 µm vibratome sections obtained from paraformaldehyde fixed brains
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Christoph Schwarzer
Verified customer
投稿 Nov 03 2006