Biotin Anti-BrdU 抗体 (ab2284)

Reviews (6)Q&A (7)References (42)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (ab2284)
  • Immunocytochemistry/ Immunofluorescence - Biotin Anti-BrdU antibody (ab2284)
  • Immunohistochemistry (Frozen sections) - Biotin Anti-BrdU antibody (ab2284)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (ab2284)

Key features and details

  • Biotin Sheep polyclonal to BrdU
  • Suitable for: IHC-P, IHC-Fr, ICC/IF, ELISA, IHC-FoFr
  • Reacts with: Species independent
  • Conjugation: Biotin
  • Isotype: IgG

製品の概要

  • 製品名

    Biotin Anti-BrdU antibody
    BrdU 一次抗体 製品一覧

  • 製品の詳細

    Biotin Sheep polyclonal to BrdU

  • 由来種

    Sheep

  • 標識

    Biotin

  • アプリケーション

    適用あり: IHC-P, IHC-Fr, ICC/IF, ELISA, IHC-FoFrmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH)

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2284の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (3)
Use at an assay dependent concentration.
IHC-Fr (1)
Use at an assay dependent concentration. Fixation in cold methanol for 30 minutes followed by immersion in 7 x 10-3 N NaOH for 10-15 seconds allows BrdU staining with the simultaneous detection of nuclear cytoplasmic and membrane assigns as well as preservation of morphological detail.
ICC/IF (1)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration. dilute from 1/500 to 1/40,000 against 1mg/mL BrdU analyte.
IHC-FoFr (1)
1/2000. 1/2000 (see Abreview).
特記事項
IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration. Fixation in cold methanol for 30 minutes followed by immersion in 7 x 10-3 N NaOH for 10-15 seconds allows BrdU staining with the simultaneous detection of nuclear cytoplasmic and membrane assigns as well as preservation of morphological detail.
ICC/IF
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration. dilute from 1/500 to 1/40,000 against 1mg/mL BrdU analyte.
IHC-FoFr
1/2000. 1/2000 (see Abreview).

ターゲット情報

  • 関連性

    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

  • 細胞内局在

    Nuclear

  • 別名

    • Bromodeoxyuridine antibody
    • BUdr antibody

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (ab2284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (ab2284)This image is courtesy of an anonymous Abreview

    ab2284 staining BrdU in mouse intestine tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% H2O2 in methanol for 12 minutes; antigen retrieval was by heat mediation in 10mM citrate, pH6. Samples were incubated with primary antibody (1/100) for 24 hours at 4°C.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Biotin Anti-BrdU antibody (ab2284)
    Immunocytochemistry/ Immunofluorescence - Biotin Anti-BrdU antibody (ab2284)This image is courtesy of an anonymous Abreview

    ab2284 at 1/250 staining primary E12 mouse cortex cells by ICC/IF. The cells were paraformaldehyde fixed, blocked with serum and then incubated with the antibody for 24 hours. Streptavidin conjugated to Alexa-Fluor ®  488 was used as the secondary. The image shows BrdU staining with nuclei counterstained with DAPI.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Biotin Anti-BrdU antibody (ab2284)
    Immunohistochemistry (Frozen sections) - Biotin Anti-BrdU antibody (ab2284)This image is courtesy of an Abreview submitted by Ahmar Aziz

    ab2284 staining BrdU in Mouse skin tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked with 10% serum for 30 minutes. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 4°C. A Streptavidin Alexa Fluor® 488-conjugated Goat polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (ab2284)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-BrdU antibody (ab2284)This image is courtesy of an Abreview submitted by Dr Christoph Schwarzer
    ab2284 at 1/2000 dilution staining mouse free floating brain slices by Immunohistochemistry (Formalin/PFA fixed sections). The mice were treated with 100mg/kg BrdU 2 hours before fixation. Free floating 40µm vibratome sections were obtained from paraformaldehyde fixed brains, these were incubated with the antibody for 24 hours. A streptavidin-HRP complex and DAB were used for detection. The image depicts the subventricular zone.

    See Abreview

参考文献 (42)

ab2284 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2284 は 42 報の論文で使用されています。

  • Fu DJ  et al. Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5-CD44+ cells. Nat Commun 11:84 (2020). PubMed: 31901081
  • Xiao Y  et al. LncRNA MALAT1 increases the stemness of gastric cancer cells via enhancing SOX2 mRNA stability. FEBS Open Bio 9:1212-1222 (2019). PubMed: 31037832
  • Flentke GR  et al. Alcohol-mediated calcium signals dysregulate pro-survival Snai2/PUMA/Bcl2 networks to promote p53-mediated apoptosis in avian neural crest progenitors. Birth Defects Res N/A:N/A (2019). PubMed: 31021056
  • Razumilava N  et al. Hedgehog Signaling Modulates Interleukin-33-Dependent Extrahepatic Bile Duct Cell Proliferation in Mice. Hepatol Commun 3:277-292 (2019). PubMed: 30766964
  • Campanale JP  et al. Methods to label, isolate, and image sea urchin small micromeres, the primordial germ cells (PGCs). Methods Cell Biol 150:269-292 (2019). PubMed: 30777180
View all Publications for this product

レビューと Q&A

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1-10 of 13 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-BrdU antibody (Biotin)

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10%
Fixative
Acetone
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Ahmar Aziz

Verified customer

投稿 Feb 10 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody (Biotin)

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate, pH6
Sample
Mouse Tissue sections (intestine)
Specification
intestine
Blocking step
(agent) for 12 minute(s) · Concentration: 1%
Fixative
Formaldehyde
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Abcam user community

Verified customer

投稿 Jun 09 2014

Question

May I put this antibody in -800C for storage?

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Answer

This antibody can be at -80C in aliquots for storage, and this may extend the life of the antibody. Either -20C or -80C will be fine.

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Question

I use your protocol for BrdU staining. Please see attached file. I used your antibody, ab2284 on mice intestine tissue last November, I got beautiful staining. This month, we are doing another batch of mice and following exactly protocol as November’s. I did three run of staining to exclude any mistake I might have and still got no stain at all. Finally, I thought it was DAB problem and emailed you last week. After receiving new DAB, I repeat BrdU stain with a fresh aliquated antibody. Then I got faint stain - comparing with last November’s staining. At that time, I thought it was Streaptavidin problem and emailed you for that. Since I need result quickly, I run another run with my real slides on Monday with the antibody I thawed last Thursday (got result on Friday) and got no stain at all even with new DAB. This antibody was aliquated and stored at -200C since we received last September. This particular tube was thawed last Thursday and used only once. It was put back to -200C right after usage. This Monday was the second time to thaw and to use, and I did not get any staining, the only explanation is that the antibody got degraded.

I hope you agree with me or give me some suggestion. I also hope you could send me new antibody to see what I get.

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Answer

Thank you for your reply and forsending the additional information.

It does sound like the antibody was damaged during storage, so I am happy to send a replacement vial for you. This will be coming free of charge on the order ***, which should arrive tomorrow. Please note that we recommend storing this antibody in working aliquots, so that each aliquot is not frozen and thawed more than one time.

I apologize for the trouble with these products, but please keep me updated about how this replacement antibody works in your staining. Let me know if you have any questions or if there is anything else that we can do for you.

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Question

We want to use the biotinylated anti-BrdU antibody without a secondary antibody. I was wondering if you would suggest blocking the tissue with hydrogen peroxide or denaturing the DNA with HCl first?

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Answer

You will denature the DNA first and then carry on with the IHC staining protocol.

https://www.abcam.com/index.html?pageconfig=resource&rid=11533
https://www.abcam.com/assets/popups/popup_datasheet_protocols_printer_friendly.cfm?pid=1325&intAbId=2284

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody (Biotin)

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse tumour)
Specification
Mouse tumour
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Perkin-Elmer TNB Blocking Reagent as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Feb 22 2008

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody (Biotin)

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain,lateral ventricle)
Specification
brain,lateral ventricle
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Tris Buffered Saline- 0.3% Triton X
Permeabilization
Yes - 4N HCl
Blocking step
NDS as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 23°C
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Mr. stefano gaburro

Verified customer

投稿 Nov 01 2007

Question

Do you know if somebody try to precipitate chromatin with BrdU antibody?

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Answer

I managed to find some references that you might be interested in. 1) Hiraga,et al. 2005. DNA polymerases alpha, delta, and epsilon localize and function together at replication forks in Saccharomyces cerevisiae. Genes to Cells. 10, 297-309. (http://www.genestocellsonline.org/cgi/content/full/10/4/297) 2) Lin, et al. 2006. Inhibition of estradiol-induced mammary proliferation by dibenzoylmethane through the E2–ER–ERE-dependent pathway. Carcinogenesis. 27(1):131-136

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Immunocytochemistry/ Immunofluorescence abreview for Anti-BrdU antibody (Biotin)

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Primary E12 mouse cortex)
Specification
Primary E12 mouse cortex
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Abcam user community

Verified customer

投稿 Feb 22 2007

Question

Is the borate buffer made with just boric acid or boric acid plus Nabicarbonate? I have found to borate buffer recipies see below. I did try looking at the ref on the website from you IHC borate wash buffer but it is not accessible online. I assume the buffer is just quenching the acid? Borate buffer 1. 1M Borate Buffer 100mls: 6.183g boric acid, 70mls dh2O and ph to pH8.0 with 10N NaOH Dilute 1:10 with dH2O to give 0.1M borate buffer. 2. Boric Acid-Borate Buffer (pH 7.6) Stock Solution A: Boric Acid 1.24 gm/100 ml distilled water Stock Solution B: Sodium Biborate 1.9 gm/100 ml distilled water Solution A ......... 85 ml Solution B ......... 15 ml Mix solutions A and B. Stable at room temperature. Supplies: Boric acid (H3BO3) Mallinckrodt Specialty Chemical Co. http://www.mallinckrodt.com/ Paris, KY Phone: 1-314-654-2000 Catalog No. 2549 Sodium biborate (Na2B4O7.10H2O) J.T. Baker Inc. http://www.jtbaker.com/ Phillipsburg, NJ Phone: 1-800-582-2537 Catalog No. 3570-01 Thankyou for your help Best wishes

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Answer

Thank you for your question. I'm afraid as I have not done the experiments I don't have a recipe for the borate buffer. I find that IHC world (www.IHCworld.com) has often some good protocols, and the following link (http://www.ihcworld.com/_protocols/antibody_protocols/brdu_accurate_chem_sci.htm) mentions 0.1M Borate Buffer, pH 8.5 made with Sodium borate (MW 381.4) 3.8 g + Distilled water 100 ml. Indeed the Borate buffer is simply to neutralize the acid.

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1-10 of 13 Abreviews or Q&A

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