Biotin Anti-BAFF 抗体 (ab271214)
Key features and details
- Biotin Rabbit polyclonal to BAFF
- Suitable for: WB, ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-BAFF antibody
BAFF 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to BAFF -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant fragment corresponding to Human BAFF aa 100-300.
Database link: Q9Y275 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab271214の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 31 kDa.
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ELISA |
Use a concentration of 0.25 - 1 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 31 kDa. |
ELISA
Use a concentration of 0.25 - 1 µg/ml. |
ターゲット情報
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機能
Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B-and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. -
組織特異性
Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. -
配列類似性
Belongs to the tumor necrosis factor family. -
翻訳後修飾
The soluble form derives from the membrane form by proteolytic processing.
N-glycosylated. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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別名
- ApoL related ligand TALL 1 antibody
- B cell Activating Factor antibody
- B lymphocyte stimulator antibody
see all
画像
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Western blot analysis of varying levels of Recombinant human BAFF protein using ab271214 at 0.1 µg/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant BAFF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Lane ng/lane
1 Marker
2 250
3 125
4 62.5
5 31.25
6 15.625
7 7.8
8 3.9
9 1.95
10 0.975
11 0.4875
12 0.24 -
Human BAFF was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271214. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 1.5-3.0 ng/lane of recombinant BAFF protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab271214 は論文での使用が確認できていません。