製品の概要

  • 製品名
    Anti-Biotin antibody (Texas Red ®)
    Biotin 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Biotin (Texas Red ®)
  • 由来種
    Goat
  • 標識
    Texas Red ®. Ex: 596nm, Em: 620nm
  • アプリケーション
    適用あり: Flow Cyt, Immunomicroscopymore details
  • 免疫原

    Chemical/ Small Molecule conjugated to KLH.

  • 特記事項

    Texas Red™ is a registered trademark of Molecular Probes Inc.

    Texas Red™ Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt
Immunomicroscopy
  • 追加情報
    suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
  • ターゲット情報

    • 関連性
      Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
    • 別名
      • Vitamin B4 antibody

    プロトコール

    参考文献

    ab6653 has not yet been referenced specifically in any publications.

    レビューと Q&A

    1-3 of 3 Q&A

    Answer

    There are still free epitopes available for a secondary antibody to bind. But you could also use an unconjugated secondary antibody.

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    Question

    Hi Jennifer Nice to talk with you. Please see the below answers attached to each questions what you had. Please reply ASAP.Thanks! Have a nice day! technical@abcam.com wrote: Dear Wenxin, I have some general questions, the answers to which will enable me to investigate this matter as quickly as possible. 1. Please describe the problem (high background, no staining etc). The particulate precipitate was observed from antibody-solution in the vial and also was found in the nodules section under confocal microscope. 2. On what material are you testing the antibody in IHC? •Species? Soybean (Glycine max) •Cell line? •Tissue? Nodules 3. How did you fix the samples? •Ethanol, methanol •Acetone •Paraformaldehyde and Other FAA 4. Did you apply antigen retrieval step? No •Enzymatic method •Heat mediated technique •Other 5. How did you block the unspecific binding sites? 3% BSA in TBST buffer 6. Primary antibody •Specification (in which species was it raised against)? Products was labeled with Biotin •At what dilution(s) have you tested this antibody? 1: 100 •Incubation time, wash steps (multiple short washes are more effective than fewer longer wash steps)? 1-2 hr at RT; 2 x 5 min with TBST buffer 7. Secondary antibody NO •What secondary antibody are you using? •Specification (in which species was it raised against)? •At what dilution(s) have you tested this antibody? •Incubation, wash steps? •Do you know whether the problems you are experiencing come from the secondary? •What detection method are you using? 8. Background staining •Please provide an image of your staining we have no result for this experiment for several times but we have very nice data when we use Dig-system. 9. Which detection system did you use? confocal microscope 10. Did you apply positive and negative controls along with the samples? Please specify. Yes, no products were generated under negative control. For positive, we used biotin-anti--Dig. 11. Optimization attempts •How many times have you tried the IHC? Three times for each fixed sample (FAA or Paraformaldehyde) •Do you obtain the same results every time? Yes •What steps have you altered? No

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    Answer

    Thank you very much for the information that you have provided. You should not have precipitate in your vial and so I'm sending you a replacement vial free of charge. For your record it is order# 39078 and you should receive it Tuesday or Wednesday of next week. Please let me know how the replacement works out for you.

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    Answer

    The molar ratios of dye-to-protein are varied batch to batch. The general acceptance criteria for this product is 2-5:1

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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