Application
Western blot
Sample
Human Tissue lysate - whole (Ovarian tissue)
Loading amount
50 µg
Specification
Ovarian tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/10000
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 66 kDa
Positive control
Serum
Negative control
serum, no primary antibody
Additional data
Additional Notes
50 ug of biotinylated-tissue was immunoprecipitated with MAH-Albumin IgG. Western blot was probed with anti-biotin pAb and with streptavidin-HRP as secondary antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. John Andersen
Verified customer
投稿 Sep 13 2006