Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse ES cells)
Specification
mouse ES cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Other product details
Dilution
1/100
Incubation time
4 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/200
Additional data
Additional Notes
This antibody had a lot of unspecific binding, which was confirmed by protein knockdown. The protein of interest was biotinalyted.
Abcam response
Thank you for your feedback. We are very sorry that this antibody did not work in your hands. Due to the nature of antibodies, protocol optimization sometime are necessary for an antibody to work in experiments. We have successfully used this antibody in WB and the image is published on the datasheet. We are sure that with some troubleshooting in protocol the antibody will be absolutely fine to use. Contact Abcam technical team for more information.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 30 2012