Application
Immunoprecipitation
Sample
Mouse Cell lysate - nuclear (mouse liver)
Total protein in input
50 µg
Specification
mouse liver
Immuno-precipitation step
Protein A/G
Other product details
Concentration
2 µg/mg lysate
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Tris-Nacl Buffer
Western Blot antibody
Name
Non-Abcam antibody was used: anti-avadin
Host species: Rat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Additional data
Additional Notes
This antibody has specific binding for Biotin, which works well for IP on the protein of interest. The experiment was followed by western on the tagged interacting protein (tagged with His). The antibody works fine, but because of the non-specific binding, it does not work well well for western for my purpose.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 30 2012