Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse liver)
Loading amount
50 µg
Specification
mouse liver
Gel Running Conditions
Reduced Denaturing (8% Precast Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-T
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 40 kDa Non-specific: 95, 120 kDa
Additional data
Additional Notes
This antibody does not work well for me because of the background, since a lot of protein (of my interest) have molecular weight in the range on 80 to 120, the background from this antibody makes this antibody not a good choice for me.
Abcam response
Thank you for your feedback. We are very sorry that this antibody did not work in your hands. Due to the nature of antibodies, protocol optimization sometime are necessary for an antibody to work in experiments. We have successfully used this antibody in WB and the image is published on the datasheet. We are sure that with some troubleshooting in protocol the antibody will be absolutely fine to use.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jan 23 2012