Biotin 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to Biotin
アプリケーション適用あり: IHC-P, ICC/IF, WB, ELISA, IPmore details
種交差性交差種: Species independent
Rabbits were immunized with KLH-Biotin After multiple immunizationsin Freund's adjuvant the rabbits were bled and serum collected.
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファーPreservative: 0.1% Sodium azide
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精製度Immunogen affinity purified
特記事項（精製）The antibodies were isolated using affinity chromatography with Lysine-Biotin as the ligand.
Our Abpromise guarantee covers the use of ab1227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/2000.|
|ICC/IF||1/200 - 1/2000.|
|WB||1/1000 - 1/30000.|
|ELISA||1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000.|
|IP||Use at an assay dependent concentration.|
関連性Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
- Vitamin B4 antibody
Anti-Biotin antibody (ab1227) at 1/10000 dilution + 50ug biotinylated human Ovarian tissue.
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
This product has been referenced in:
- Villella VR et al. A pathogenic role for cystic fibrosis transmembrane conductance regulator in celiac disease. EMBO J 38:N/A (2019). Read more (PubMed: 30498130) »
- Bollong MJ et al. A metabolite-derived protein modification integrates glycolysis with KEAP1-NRF2 signalling. Nature 562:600-604 (2018). Read more (PubMed: 30323285) »