Anti-Biotin 抗体 (ab1227)
Key features and details
- Rabbit polyclonal to Biotin
- Suitable for: ICC/IF, WB, IHC, ELISA, IP
- Reacts with: Species independent
- Isotype: IgG
製品の概要
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製品名
Anti-Biotin antibody
Biotin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Biotin -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC, ELISA, IPmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule corresponding to Biotin conjugated to keyhole limpet haemocyanin. Rabbits were immunized with KLH-Biotin. After multiple immunizations in Freund's adjuvant the rabbits were bled and serum collected.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.1% Sodium azide -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibodies were isolated using affinity chromatography with Lysine-Biotin as the ligand. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab1227の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | (2) |
1/200 - 1/2000.
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| WB | (3) |
1/1000 - 1/30000.
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| IHC |
1/200 - 1/2000.
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| ELISA |
1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000.
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| IP | (2) |
Use at an assay dependent concentration.
|
| 特記事項 |
|---|
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ICC/IF
1/200 - 1/2000. |
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WB
1/1000 - 1/30000. |
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IHC
1/200 - 1/2000. |
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ELISA
1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000. |
|
IP
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
別名
- Vitamin B4 antibody
画像
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Western blot - Anti-Biotin antibody (ab1227)This image is courtesy of an Abreview submitted by Mr John AndersenAnti-Biotin antibody (ab1227) at 1/10000 dilution + 50ug biotinylated human Ovarian tissue.
Secondary
Streptavidin-HRP
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab1227 は 13 報の論文で使用されています。
- Chen G et al. bFGF alleviates diabetes-associated endothelial impairment by downregulating inflammation via S-nitrosylation pathway. Redox Biol 41:101904 (2021). PubMed: 33706169
- He J et al. An R2R3 MYB transcription factor confers brown planthopper resistance by regulating the phenylalanine ammonia-lyase pathway in rice. Proc Natl Acad Sci U S A 117:271-277 (2020). PubMed: 31848246
- Fontana GA et al. Rif1 S-acylation mediates DNA double-strand break repair at the inner nuclear membrane. Nat Commun 10:2535 (2019). PubMed: 31182712
- Esposito S et al. Genistein antagonizes gliadin-induced CFTR malfunction in models of celiac disease. Aging (Albany NY) 11:2003-2019 (2019). PubMed: 30981209
- Villella VR et al. A pathogenic role for cystic fibrosis transmembrane conductance regulator in celiac disease. EMBO J 38:N/A (2019). PubMed: 30498130