製品の概要
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製品名Anti-Biotin antibody
Biotin 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Biotin
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由来種Rabbit
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アプリケーション適用あり: IHC-P, ICC/IF, WB, ELISA, IPmore details
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免疫原
Rabbits were immunized with KLH-Biotin After multiple immunizationsin Freund's adjuvant the rabbits were bled and serum collected.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPBS with 0.1% sodium azide
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Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）The antibodies were isolated using affinity chromatography with Lysine-Biotin as the ligand.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab1227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/200 - 1/2000. | |
| ICC/IF | 1/200 - 1/2000. | |
| WB | 1/1000 - 1/30000. | |
| ELISA | 1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000. | |
| IP | Use at an assay dependent concentration. |
ターゲット情報
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関連性Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
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別名
- Vitamin B4 antibody
画像
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Western blot - Anti-Biotin antibody (ab1227)This image is courtesy of an Abreview submitted by Mr John AndersenAnti-Biotin antibody (ab1227) at 1/10000 dilution + 50ug biotinylated human Ovarian tissue.
Secondary
Streptavidin-HRP
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
プロトコール
参考文献
This product has been referenced in:
- Banuelos CA et al. Sintokamide A Is a Novel Antagonist of Androgen Receptor That Uniquely Binds Activation Function-1 in Its Amino-terminal Domain. J Biol Chem 291:22231-22243 (2016). Read more (PubMed: 27576691) »
- Waaijers S et al. A tissue-specific protein purification approach in Caenorhabditis elegans identifies novel interaction partners of DLG-1/Discs large. BMC Biol 14:66 (2016). WB . Read more (PubMed: 27506200) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"