Anti-Biotin 抗体 (ab1227)
Key features and details
- Rabbit polyclonal to Biotin
- Suitable for: IHC-P, ICC/IF, WB, ELISA, IP
- Reacts with: Species independent
- Isotype: IgG
製品の概要
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製品名
Anti-Biotin antibody
Biotin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Biotin -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WB, ELISA, IPmore details -
種交差性
交差種: Species independent -
免疫原
Rabbits were immunized with KLH-Biotin After multiple immunizationsin Freund's adjuvant the rabbits were bled and serum collected.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.1% Sodium azide -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
The antibodies were isolated using affinity chromatography with Lysine-Biotin as the ligand. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab1227 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/200 - 1/2000. | |
| ICC/IF | 1/200 - 1/2000. | |
| WB | 1/1000 - 1/30000. | |
| ELISA | 1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000. | |
| IP | Use at an assay dependent concentration. |
ターゲット情報
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関連性
Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da. -
別名
- Vitamin B4 antibody
画像
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Western blot - Anti-Biotin antibody (ab1227)This image is courtesy of an Abreview submitted by Mr John AndersenAnti-Biotin antibody (ab1227) at 1/10000 dilution + 50ug biotinylated human Ovarian tissue.
Secondary
Streptavidin-HRP
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
プロトコール
参考文献 (11)
ab1227 は 11 報の論文で使用されています。
- Villella VR et al. A pathogenic role for cystic fibrosis transmembrane conductance regulator in celiac disease. EMBO J 38:N/A (2019). PubMed: 30498130
- Fontana GA et al. Rif1 S-acylation mediates DNA double-strand break repair at the inner nuclear membrane. Nat Commun 10:2535 (2019). PubMed: 31182712
- Esposito S et al. Genistein antagonizes gliadin-induced CFTR malfunction in models of celiac disease. Aging (Albany NY) 11:2003-2019 (2019). PubMed: 30981209
- Wang J et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
- Bollong MJ et al. A metabolite-derived protein modification integrates glycolysis with KEAP1-NRF2 signalling. Nature 562:600-604 (2018). PubMed: 30323285
