製品の概要

  • 製品名

  • 製品の詳細

    Rabbit polyclonal to Biotin
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-P, ICC/IF, WB, ELISA, IPmore details
  • 種交差性

    交差種: Species independent
  • 免疫原

    Rabbits were immunized with KLH-Biotin After multiple immunizationsin Freund's adjuvant the rabbits were bled and serum collected.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/2000.
ICC/IF 1/200 - 1/2000.
WB 1/1000 - 1/30000.
ELISA 1/100 - 1/1000. (Coating). For primary: 1/1000 - 1/30,000.
IP Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • 別名

    • Vitamin B4 antibody

画像

  • Anti-Biotin antibody (ab1227) at 1/10000 dilution + 50ug biotinylated human Ovarian tissue.

    Secondary
    Streptavidin-HRP

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds

    See Abreview

参考文献

This product has been referenced in:

  • Villella VR  et al. A pathogenic role for cystic fibrosis transmembrane conductance regulator in celiac disease. EMBO J 38:N/A (2019). Read more (PubMed: 30498130) »
  • Bollong MJ  et al. A metabolite-derived protein modification integrates glycolysis with KEAP1-NRF2 signalling. Nature 562:600-604 (2018). Read more (PubMed: 30323285) »
See all 8 Publications for this product

レビューと Q&A

1-7 of 7 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (293 cells)
Specification
293 cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Abcam user community

Verified customer

投稿 Jan 30 2012

Application
Immunoprecipitation
Sample
Mouse Cell lysate - nuclear (mouse liver)
Total protein in input
50 µg
Specification
mouse liver
Immuno-precipitation step
Protein A/G

Abcam user community

Verified customer

投稿 Jan 30 2012

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (mouse ES cells)
Specification
mouse ES cells
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Abcam user community

Verified customer

投稿 Jan 30 2012

Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse liver)
Loading amount
50 µg
Specification
mouse liver
Gel Running Conditions
Reduced Denaturing (8% Precast Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Jan 23 2012

Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (HEK293)
Total protein in input
2000 µg
Specification
HEK293
Immuno-precipitation step
Other - Protein G Dynabeads

Abcam user community

Verified customer

投稿 Dec 06 2011

Application
Western blot
Sample
Human Cell lysate - nuclear (HEK293-T)
Loading amount
100 µg
Specification
HEK293-T
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Dec 06 2011

Application
Western blot
Sample
Human Tissue lysate - whole (Ovarian tissue)
Loading amount
50 µg
Specification
Ovarian tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Mr. John Andersen

Verified customer

投稿 Sep 13 2006

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