製品の概要

  • 製品名
    Anti-Biotin antibody [39-15D9]
    Biotin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [39-15D9] to Biotin
  • 由来種
    Mouse
  • 特異性
    This antibody reacts with both free biotin and biotin bound to a carrier protein. There is no cross reaction with the free carrier protein (determined by ELISA).
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 免疫原

    Full length protein conjugated to KLH.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000 - 1/10000.
WB 1/1000 - 1/5000.

ターゲット情報

  • 関連性
    Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
  • 別名
    • Vitamin B4 antibody

プロトコール

参考文献

This product has been referenced in:
  • Potluri LP  et al. ZipA is required for FtsZ-dependent preseptal peptidoglycan synthesis prior to invagination during cell division. J Bacteriol 194:5334-42 (2012). Read more (PubMed: 22843850) »
  • Ballard PL  et al. Regulated gene expression in cultured type II cells of adult human lung. Am J Physiol Lung Cell Mol Physiol 299:L36-50 (2010). Read more (PubMed: 20382749) »

See all 2 Publications for this product

ab3780 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

4 reviews or Q&A

10
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Will this antibody bind the d-isomer of biotin?

Thank you for your enquiry. This antibody does bind the d-isomer of biotin. Please do not hesitate to contact us if you have additional questions.

I am interested in your anti Biotin antibody (ab3780). Is there any Reference for this product? Where does the hybridoma cell line come from?

Thank you for your enquiry. The hybridoma cell line comes from the antibody's originator (the source's name is proprietary) and unfortunately we do not have any references at this time. If you have any additional questions, please contact us again.

Having ordered your anti biotin antibody(ab3780) to use in a protein capture experiment,I find no information on the data sheet as to the concentration of the immunoglobulin in the ascites fluid.Is this just an omission? This makes quantitation of my e...

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Thank you for your enquiry. We are sorry to inform you that there is no concentration of the immunoglobulin for antibodies that are not affinity purified. Please take a look at the suggested dilutions for each application listed on the datasheet.

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what size should biotin be on a western blot

On Western blot membran you can detect biotinylated proteins but not free biotine. Therefore the size of the bands depends of the molecular weight of the biotintylated protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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