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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-BIN1 抗体 [EPR13463-25] - BSA and Azide free (ab226006)

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Western blot - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)
  • Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)
  • Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)
  • Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)
  • Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13463-25] to BIN1 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Unconjugated

製品の概要

  • 製品名

    Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free
    BIN1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13463-25] to BIN1 - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, IHC-P, WB, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • U87-MG, Human fetal kidney and Hela cell lysates; Human fetal brain lysate;, Human skeletal muscle and mouse brain tissues; Hela and U87-MG cells.

  • 特記事項

    ab226006 is the carrier-free version of ab185950.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab226006の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 56, 65, 70, 90 kDa (predicted molecular weight: 65 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
特記事項
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 56, 65, 70, 90 kDa (predicted molecular weight: 65 kDa).
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.

  • 組織特異性

    Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.

  • 関連疾患

    Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.

  • 配列類似性

    Contains 1 BAR domain.
    Contains 1 SH3 domain.

  • 翻訳後修飾

    Phosphorylated by protein kinase C.

  • 細胞内局在

    Cytoplasm and Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AMPH 2 antibody
    • AMPH2 antibody
    • Amphiphysin 2 antibody
    • Amphiphysin II antibody
    • Amphiphysin like protein antibody
    • amphiphysin-like antibody
    • Amphiphysin-like protein antibody
    • AMPHL antibody
    • Bin1 antibody
    • BIN1_HUMAN antibody
    • Box Dependant MYC Interacting Protein 1 antibody
    • Box-dependent myc-interacting protein 1 antibody
    • Bridging integrator 1 antibody
    • DKFZp547F068 antibody
    • MGC10367 antibody
    • MGC105358 antibody
    • Myc box dependent interacting protein 1 antibody
    • Myc box-dependent-interacting protein 1 antibody
    • SH3P9 antibody
    see all

画像

  • Western blot - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)
    Western blot - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)

    This WB data was generated using the same anti-BIN1 antibody clone [EPR13463-25] in a different buffer formulation (cat# ab185950).

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: BIN1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: U-87MG whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab185950 observed at 45-65 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab185950 was shown to specifically react with BIN1 when BIN1 knockout samples were used. Wild-type and BIN1 knockout samples were subjected to SDS-PAGE. Ab185950 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)
    Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)

    Intracellular flow cytometric analysis of Hela cells labeling BIN1 using ab185950 at 1/90 dilution (red). Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG (green). 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185950). 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)
    Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)

    Immunofluorescent analysis of U87-MG cells labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi (blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185950).

  • Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)
    Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)

    Immunofluorescent analysis of HeLa cells labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: -20? Acetone. Counter stain: Dapi (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185950).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185950).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185950).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)
    Anti-BIN1 antibody [EPR13463-25] - BSA and Azide free (ab226006)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab226006 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab226006 は論文での使用が確認できていません。

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