遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-BIN1 抗体 [EPR13463-25] (ab185950)

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Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
  • Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)
  • Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
  • Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)
  • Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)
  • Anti-BIN1 antibody [EPR13463-25] (ab185950)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13463-25] to BIN1
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-BIN1 antibody [EPR13463-25]
    BIN1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13463-25] to BIN1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • U87-MG, Human fetal kidney and HeLa whole cell lysate (ab150035); Human fetal brain lysate;, Human skeletal muscle and mouse brain tissues; HeLa and U87-MG cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185950の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
1/100.
WB (1)
1/1000. Detects a band of approximately 56, 65, 70, 90 kDa (predicted molecular weight: 65 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
特記事項
ICC/IF
1/100.
WB
1/1000. Detects a band of approximately 56, 65, 70, 90 kDa (predicted molecular weight: 65 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/90.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能

    May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.

  • 組織特異性

    Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.

  • 関連疾患

    Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.

  • 配列類似性

    Contains 1 BAR domain.
    Contains 1 SH3 domain.

  • 翻訳後修飾

    Phosphorylated by protein kinase C.

  • 細胞内局在

    Cytoplasm and Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • AMPH 2 antibody
    • AMPH2 antibody
    • Amphiphysin 2 antibody
    • Amphiphysin II antibody
    • Amphiphysin like protein antibody
    • amphiphysin-like antibody
    • Amphiphysin-like protein antibody
    • AMPHL antibody
    • Bin1 antibody
    • BIN1_HUMAN antibody
    • Box Dependant MYC Interacting Protein 1 antibody
    • Box-dependent myc-interacting protein 1 antibody
    • Bridging integrator 1 antibody
    • DKFZp547F068 antibody
    • MGC10367 antibody
    • MGC105358 antibody
    • Myc box dependent interacting protein 1 antibody
    • Myc box-dependent-interacting protein 1 antibody
    • SH3P9 antibody
    see all

画像

  • Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: BIN1 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: U-87MG whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab185950 observed at 45-65 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab185950 was shown to specifically react with BIN1 when BIN1 knockout samples were used. Wild-type and BIN1 knockout samples were subjected to SDS-PAGE. Ab185950 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)

    Immunofluorescent analysis of HeLa cells labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: -20℃ Acetone. Counter stain: Dapi (blue).

  • Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Flow Cytometry (Intracellular) - Anti-BIN1 antibody [EPR13463-25] (ab185950)

    Intracellular flow cytometric analysis ofHela cells labeling BIN1 using ab185950 at 1/90 dilution (red). Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG (green). 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BIN1 antibody [EPR13463-25] (ab185950)

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Ready to use HRP Polymer for Rabbit IgG. Counter stain: Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Western blot - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    All lanes : Anti-BIN1 antibody [EPR13463-25] (ab185950) at 1/1000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Hela cell lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 65 kDa



    Blocking buffer: 5% NFDM/TBST

  • Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Immunocytochemistry/ Immunofluorescence - Anti-BIN1 antibody [EPR13463-25] (ab185950)

    Immunofluorescent analysis of U87-MG cells labeling BIN1 with ab185950 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi (blue)

  • Anti-BIN1 antibody [EPR13463-25] (ab185950)
    Anti-BIN1 antibody [EPR13463-25] (ab185950)

参考文献 (3)

ab185950 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185950 は 3 報の論文で使用されています。

  • Zheng J  et al. microRNA-211 promotes proliferation, migration, and invasion ability of oral squamous cell carcinoma cells via targeting the bridging integrator 1 protein. J Cell Biochem 120:4644-4653 (2019). PubMed: 30260023
  • Andrew RJ  et al. Reduction of the expression of the late-onset Alzheimer's disease (AD) risk-factor BIN1 does not affect amyloid pathology in an AD mouse model. J Biol Chem 294:4477-4487 (2019). PubMed: 30692199
  • McKenzie AT  et al. Multiscale network modeling of oligodendrocytes reveals molecular components of myelin dysregulation in Alzheimer's disease. Mol Neurodegener 12:82 (2017). WB, IHC-P ; Mouse . PubMed: 29110684

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1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-BIN1 antibody [EPR13463-25]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Heart)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
Heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Aug 17 2020

Immunohistochemistry (Frozen sections) abreview for Anti-BIN1 antibody [EPR13463-25]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Aug 11 2020

Immunohistochemistry (Frozen sections) abreview for Anti-BIN1 antibody [EPR13463-25]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Jun 07 2016

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