Anti-Bik 抗体 (ab62494)
Key features and details
- Rabbit polyclonal to Bik
- Suitable for: WB, ICC, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Bik antibody
Bik 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Bik -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Bik (N terminal). 15 amino acid synthetic peptide from near the amino terminus
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ポジティブ・コントロール
- Jurkat whole cell lysate (ab7899) and Jurkat cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62494の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 18 kDa).
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ICC |
Use a concentration of 1 - 20 µg/ml.
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ICC/IF |
Use a concentration of 1 - 20 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 18 kDa). |
ICC
Use a concentration of 1 - 20 µg/ml. |
ICC/IF
Use a concentration of 1 - 20 µg/ml. |
ターゲット情報
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機能
Accelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX. -
ドメイン
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
細胞内局在
Endomembrane system. Around the nuclear envelope, and in cytoplasmic membranes. - Information by UniProt
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参照データベース
- Entrez Gene: 638 Human
- Omim: 603392 Human
- SwissProt: Q13323 Human
- Unigene: 475055 Human
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別名
- Apoptosis inducer NBK antibody
- BBC1 antibody
- Bcl-2-interacting killer antibody
see all
画像
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Lane 1 : Anti-Bik antibody (ab62494) at 1 µg/ml
Lane 2 : Anti-Bik antibody (ab62494) at 2 µg/ml
All lanes : Jurkat cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 18 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted? -
Immunocytochemistry of Bik in Jurkat cells with ab62494 antibody at 1 µg/ml.
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Immunofluorescence of Bik in Jurkat cells using ab62494 at 10 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab62494 は論文での使用が確認できていません。