Anti-Bid Cleavage Site 抗体 (ab10640)
Key features and details
- Rabbit polyclonal to Bid Cleavage Site
- Suitable for: ICC, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Bid Cleavage Site antibody -
製品の詳細
Rabbit polyclonal to Bid Cleavage Site -
由来種
Rabbit -
アプリケーション
適用あり: ICC, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Mouse Bid Cleavage Site (N terminal). Synthetic peptide (Mouse)corresponding to N-terminus of cleavage site (59/60).
Database link: P70444 -
ポジティブ･コントロール
- WB: 3T3-L1 cells extracts. ICC: A549 cells.
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特記事項
BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl 2 family. BID interacts with both Bcl 2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from rabbit serum by epitope-specific affinity chromatography. -
一次抗体 備考
BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl 2 family. BID interacts with both Bcl 2 and Bax through its BH3 domain. It usually exists in an inactive form in the cytosolic fraction of living cells and becomes cleaved and activated by caspase 8 in response to TNF alpha or Fas ligand. Once BID is cleaved, the C-terminal 15 kDa fragment of BID (p15) translocates onto mitochondria and is sufficient to trigger cytochrome c release, resulting in cell apoptosis. BID serves as a direct molecular link between caspase 8 activation and mitochondrial death machinery. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10640の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
1/250.
|
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| WB | (1) |
1/1000. Predicted molecular weight: 15 kDa.
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| 特記事項 |
|---|
|
ICC
1/250. |
|
WB
1/1000. Predicted molecular weight: 15 kDa. |
ターゲット情報
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関連性
Bid, a BH3 domain containing proapoptotic Bcl2 family member, is localized in the cytosolic fraction of cells as an inactive precursor. Its active form is generated upon proteolytic cleavage by caspase 8 in the Fas signaling pathway. Cleaved Bid translocates to mitochondria and releases its potent proapoptotic activity, which in turn induces cytochrome c release and mitochondrial damage. The cytochrome c releasing activity of Bid was antagonized by Bcl2. Mutation in the SH3 domain can diminish the cytochrome c releasing activity. In animal model studies, Bid deficient mice are found resistant to the lethal effects of death factor signals relayed through Fas. -
参照データベース
- Entrez Gene: 637 Human
- Entrez Gene: 12122 Mouse
- Omim: 601997 Human
- SwissProt: P55957 Human
- SwissProt: P70444 Mouse
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別名
- Apoptic death agonist antibody
- BH 3 interacting domain death agonist antibody
- BH3 interacting domain death agonist antibody
see all
画像
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Western blot - Anti-Bid Cleavage Site antibody (ab10640)All lanes : Anti-Bid Cleavage Site antibody (ab10640) at 1/1000 dilution
Lane 1 : 3T3-L1 cells without BID
Lane 2 : 3T3-L1 cells with caspase-8 cleaved recombinant mouse BID
Lane 3 : 3T3-L1 cells with caspase-8 cleaved recombinant human BID
Predicted band size: 15 kDaSDS-PAGE on a 4-20% Tris-glycine gel
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Immunocytochemistry - Anti-Bid Cleavage Site antibody (ab10640)A549 cells stained for BID cleave site (green) using ab10640 at 1/250 dilution in ICC/IF. It was followed by Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at 1/400 dilution for 30 minutes at room temperature (Panel a). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant DAPI. F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e shows no primary antibody control.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (14)
ab10640 は 14 報の論文で使用されています。
- Zhang Y et al. Genetically engineered magnetic nanocages for cancer magneto-catalytic theranostics. Nat Commun 11:5421 (2020). PubMed: 33110072
- Sheng H et al. Liriopesides B induces apoptosis and cell cycle arrest in human non-small cell lung cancer cells. Int J Mol Med 46:1039-1050 (2020). PubMed: 32705266
- Chung TW et al. Antitumor effect of kurarinone and underlying mechanism in small cell lung carcinoma cells. Onco Targets Ther 12:6119-6131 (2019). PubMed: 31496721
- Li X et al. A novel cell-penetrating peptide protects against neuron apoptosis after cerebral ischemia by inhibiting the nuclear translocation of annexin A1. Cell Death Differ 26:260-275 (2019). PubMed: 29769639
- Yen JH et al. Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone. J Exp Clin Cancer Res 38:42 (2019). PubMed: 30691497