Anti-BICD1 抗体 [EPR11642] (ab170878)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11642] to BICD1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-BICD1 antibody [EPR11642]
BICD1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11642] to BICD1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IPmore details
適用なし: Flow Cyt or IHC-P -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment within Human BICD1. The exact sequence is proprietary.
Database link: Q96G01 -
ポジティブ・コントロール
- Daudi, HeLa, Jurkat and 293T cell lysates, HeLa cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR11642 -
アイソタイプ
IgG
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab170878の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 110 kDa.
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ICC/IF |
1/50 - 1/100.
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IP |
1/10 - 1/100.
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特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 110 kDa. |
ICC/IF
1/50 - 1/100. |
IP
1/10 - 1/100. |
ターゲット情報
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機能
Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport by recruiting the dynein-dynactin motor complex. -
組織特異性
Expressed in the brain, heart and skeletal muscle. -
配列類似性
Belongs to the BicD family. -
翻訳後修飾
Isoform 3 is phosphorylated on Ser-865 and Thr-866. -
細胞内局在
Golgi apparatus. - Information by UniProt
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参照データベース
- Entrez Gene: 636 Human
- Entrez Gene: 12121 Mouse
- Entrez Gene: 362466 Rat
- Omim: 602204 Human
- SwissProt: Q96G01 Human
- SwissProt: Q8BR07 Mouse
- Unigene: 505202 Human
- Unigene: 177181 Mouse
see all -
別名
- Bic-D 1 antibody
- Bicaudal D homolog 1 antibody
- BICD antibody
see all
画像
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Western blot analysis on immunoprecipitation pellet from 293T cell lysate labeling BICD1 using ab170878 at a 1/10 dilution.
Anti-BICD1 antibody [EPR11642] (ab170878) at 1/10 dilution + pellet from 293T cell lysate -
Immunofluorescent staining of HeLa cells labeling BICD1 using ab170878 at a 1/50 dilution (green) and DAPI nuclear staining (blue).
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All lanes : Anti-BICD1 antibody [EPR11642] (ab170878) at 1/1000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 110 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab170878 は 2 報の論文で使用されています。
- Homma Y & Fukuda M Knockout analysis of Rab6 effector proteins revealed the role of VPS52 in the secretory pathway. Biochem Biophys Res Commun 561:151-157 (2021). PubMed: 34023780
- Gonçalves JC et al. Nesprin-2 Recruitment of BicD2 to the Nuclear Envelope Controls Dynein/Kinesin-Mediated Neuronal Migration In Vivo. Curr Biol 30:3116-3129.e4 (2020). PubMed: 32619477