Anti-BHMT 抗体 (ab96415)
Key features and details
- Rabbit polyclonal to BHMT
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-BHMT antibody
BHMT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to BHMT -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow -
免疫原
Recombinant fragment, corresponding to a region within the internal sequence amino acids 199-393 of Human BHMT.
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ポジティブ・コントロール
- WB: HepG2 whole cell lysate (ab7900); Mouse liver, lung and heart tissue lysates SG xenograft (IHC)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96415の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 45 kDa.
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IHC-P |
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 45 kDa. |
IHC-P
1/100 - 1/500. Antigen retrieval is not essential but may optimise staining. |
ターゲット情報
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機能
Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. -
組織特異性
Found exclusively in liver and kidney. -
パスウェイ
Amine and polyamine degradation; betaine degradation; sarcosine from betaine: step 1/2.
Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (BhmT route): step 1/1. -
配列類似性
Contains 1 Hcy-binding domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 497025 Cow
- Entrez Gene: 635 Human
- Entrez Gene: 12116 Mouse
- Entrez Gene: 81508 Rat
- Omim: 602888 Human
- SwissProt: Q5I597 Cow
- SwissProt: Q93088 Human
- SwissProt: O35490 Mouse
see all -
別名
- Betaine homocysteine methyltransferase antibody
- Betaine homocysteine S methyltransferase 1 antibody
- Betaine homocysteine S methyltransferase antibody
see all
画像
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Anti-BHMT antibody (ab96415) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 45 kDa
10% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded human SG xenograft, using ab96415 at 1/100 dilution.
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All lanes : Anti-BHMT antibody (ab96415) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Mouse heart tissue lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 45 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab96415 は 5 報の論文で使用されています。
- Zhao C et al. Intrahepatic cholangiocyte regeneration from an Fgf-dependent extrahepatic progenitor niche in a zebrafish model of Alagille Syndrome. Hepatology 75:567-583 (2022). PubMed: 34569629
- Velazquez R et al. Maternal choline supplementation ameliorates Alzheimer's disease pathology by reducing brain homocysteine levels across multiple generations. Mol Psychiatry N/A:N/A (2019). PubMed: 30622336
- Carter WG et al. Isoaspartate, carbamoyl phosphate synthase-1, and carbonic anhydrase-III as biomarkers of liver injury. Biochem Biophys Res Commun 458:626-31 (2015). PubMed: 25684186
- Espe M et al. Methionine deficiency does not increase polyamine turnover through depletion of hepatic S-adenosylmethionine in juvenile Atlantic salmon. Br J Nutr 112:1274-85 (2014). PubMed: 25196630
- Zhang Y et al. Homocysteine homeostasis and betaine-homocysteine S-methyltransferase expression in the brain of hibernating bats. PLoS One 8:e85632 (2013). WB, IHC-P ; Bat . PubMed: 24376891