Application
Western blot
Sample
Mouse Tissue lysate - whole (striatum)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
striatum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 23°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 0.1% Tween-20
Dilution
1/3000
Detection
Detection method
ECL+
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Additional data
Additional Notes
A single band appears next to the 50 kDa marker. All the lanes in this blot are from the mouse striatum (non-treated mice).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alexandre Magno
Verified customer
投稿 Jul 01 2019