Application
Western blot
Sample
Rat Tissue lysate - whole (brown adipose tissue)
Gel Running Conditions
Reduced Denaturing (4-20)
Loading amount
20 µg
Treatment
in vivo delivery of viral vector to overexpress leptin in the brain
Specification
brown adipose tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/4000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: anti-Rabbit IgG- HRP linked
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL prime
Exposure
30 second(s)
Bands
Specific: 50 kDa
Positive control
brown adipose tissue
Additional data
PubMed ID
10.1139/cjpp-2016-0290: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 28 2017