Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton X100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-rabbit AlexaFluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Asynchronous cells were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces the expected cytoplasmic staining pattern for monomeric tubulin that is not incorporated into microtubules. MeOH fixed samples were also evaluated and produced a similar staining pattern.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Jan 22 2014