製品名Anti-beta Tubulin antibody
beta Tubulin 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to beta Tubulin
アプリケーション適用あり: Flow Cyt, ICC/IF, IHC-P, IP, WBmore details
種交差性交差種: Mouse, Cow, Human, Rice
交差が予測される動物種: Rat, Chicken, Guinea pig, Dog, Pig, Saccharomyces cerevisiae, Xenopus laevis, Chimpanzee, Sea urchin, Fungi, Xenopus tropicalis
Synthetic peptide within Human beta Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: Q13509
- Recombinant Human beta Tubulin protein (ab70187) can be used as a positive control in WB. MAD109 cells, Skin and lung, HeLa cell line
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab15568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 19509307
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||1/200. Antigen retrieval is not essential but may optimise staining.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.|
機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
組織特異性Ubiquitously expressed with highest levels in spleen, thymus and immature brain.
関連疾患Cortical dysplasia, complex, with other brain malformations 6
Skin creases, congenital symmetric circumferential, 1
配列類似性Belongs to the tubulin family.
ドメインThe highly acidic C-terminal region may bind cations such as calcium.
翻訳後修飾Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear.
Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.
- Information by UniProt
- Beta 4 tubulin antibody
- Beta 5 tubulin antibody
- beta Ib tubulin antibody
All lanes : Anti-beta Tubulin antibody (ab15568) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 55 kDa
Observed band size: 55 kDa
ICC/IF image of ab15568 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15568, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab15568 staining beta Tubulin in IMR32 Human neuroblastoma cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 1% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA containing TBST) for 16 hours at 4°C. A Cy3®-conjugated Goat anti-rabbit IgG polyclonal (1:1000) was used as the secondary antibody. Blue - nuclei (DAPI).
ab15568 staining beta Tubulin in Human lung by Immunohistochemistry (FFPE-sections).
This product has been referenced in:
- Wu L et al. Disulfiram and BKM120 in Combination with Chemotherapy Impede Tumor Progression and Delay Tumor Recurrence in Tumor Initiating Cell-Rich TNBC. Sci Rep 9:236 (2019). Read more (PubMed: 30659204) »
- Jing Y et al. Phosphatase Actin Regulator-1 (PHACTR-1) Knockdown Suppresses Cell Proliferation and Migration and Promotes Cell Apoptosis in the bEnd.3 Mouse Brain Capillary Endothelial Cell Line. Med Sci Monit 25:1291-1300 (2019). Read more (PubMed: 30772888) »