Anti-Beta TRCP 抗体 (ab71753)
Key features and details
- Rabbit polyclonal to Beta TRCP
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Beta TRCP antibody
Beta TRCP 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Beta TRCP -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Monkey, Orangutan -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in Caco2 whole cell lysate. This antibody gave a positive signal in the following cell types: T47D. It also gave a positive signal in FFPE human testis tissue sections.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab71753の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 68 kDa).
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ICC/IF |
Use a concentration of 5 µg/ml.
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特記事項 |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 68 kDa). |
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, SMAD3, SMAD4, CDC25A, DLG1, FBXO5 and probably NFKB2. SCF(BTRC) mediates the ubiquitination of phosphorylated SNAI1. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 F-box domain.
Contains 7 WD repeats. -
ドメイン
The N-terminal D domain mediates homodimerization. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 534072 Cow
- Entrez Gene: 8945 Human
- Entrez Gene: 12234 Mouse
- Entrez Gene: 361765 Rat
- Omim: 603482 Human
- SwissProt: Q9Y297 Human
- SwissProt: Q3ULA2 Mouse
- Unigene: 643802 Human
see all -
別名
- Beta transducin repeat containing E3 ubiquitin protein ligase antibody
- beta TrCP1 antibody
- beta-transducin repeat containing antibody
see all
画像
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Anti-Beta TRCP antibody (ab71753) at 1 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThe 62 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Human F-box/WD repeat-containing protein 1A (Beta TRCP)
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ICC/IF image of ab71753 stained T47D cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab71753 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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IHC image of Beta TRCP staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab71753, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab71753 は 11 報の論文で使用されています。
- Paul D et al. Revealing β-TrCP activity dynamics in live cells with a genetically encoded biosensor. Nat Commun 13:6364 (2022). PubMed: 36289220
- Zhang X et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics 11:5650-5674 (2021). PubMed: 33897873
- Wang Y et al. Transcriptional Repression of Ferritin Light Chain Increases Ferroptosis Sensitivity in Lung Adenocarcinoma. Front Cell Dev Biol 9:719187 (2021). PubMed: 34765600
- Parker TW & Neufeld KL APC controls Wnt-induced ß-catenin destruction complex recruitment in human colonocytes. Sci Rep 10:2957 (2020). PubMed: 32076059
- Wu D et al. MiR-135a-5p promotes the migration and invasion of trophoblast cells in preeclampsia by targeting ß-TrCP. Placenta 99:63-69 (2020). PubMed: 32758717